Abstract
Species within the genus Xanthomonas demonstrate pathogenesis to a variety of plant types, including rice, crucifers, cotton, wheat, peppers, tomatoes, and geraniums. However, Xanthomonas species do not respond well to chemical treatments that induce competence necessary for transformation studies, and a highly reproducible natural transformation system has yet to be developed. Electrotransformation, a technique by which bacteria are transformed by electroporation, offers an alternative to natural transformation systems (1). Our studies have primarily been concerned with Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight in rice (2); however, information on other species is included in our discussion.
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References
Mercenier, A., and Chassy, B. M. (1988) Strategies for the development of bacterial transformation systems. Biochimie 70, 503–517.
Jones, R. K., Barnes, L. W., Gonzalez, C. F., Leach, J. E., Alvarez, A. M., and Benedict, A. A. (1989) Identification of low virulence strains of Xanthomonas campestris pv. oryzae from rice in the USA. Phytopathology 79, 984–990.
Calvin, N. M., and Hanawalt, P. C. (1988) High-efficiency transformation of bacterial cells by electroporation. J. Bacteriol. 170, 2796–2801.
Chassy, B. M., Mercenier, A., and Flickinger, J. (1988) Transformation of bacteria by electroporation. Trends in Biotechnol. 6, 303–309.
White, T. J., and Gonzalez, C. F. (1991) Application of electroporation for efficient transformation of Xanthomonas campestris pv. oryzae. Phytopathology 81, 521–524.
Vidaver, A. K. (1967) Synthetic and complex media for the rapid detection of fluorescence of phytopathogenic pseudomonads, effect of the carbon source. Appl. Microbiol. 15, 1523,1524.
Dower, W. J., Miller, J. F., and Ragsdale, C. W. (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16, 6127–6145.
Innes, R. W., Hiroes, M. A., and Kuemple, P. L. (1988) Induction of nitrogen-fixing nodules on clover requires only 32 kilobase pairs of DNA from the Rhizobium trifolii symbiosis plasmid. J. Bacteriol. 170, 3793–3802.
Sambrook, J, Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Nordstrom, K. (1985) Replication, incompatibility and partition, in Plasmids in Bacteria (Helinski, D. R., Cohen, S. M., Clewell, D. B., Jackson, D. A., and Hollaender, A., eds.), Plenum, New York, pp. 119–123.
DeFeyter, R., Kado, C. I., and Gabriel, D. W. (1990) Small, stable shuttle vectors for use in Xanthomonas. Gene 88, 65–72.
Choi, S. H., and Leach, J. E. (1994) Identification of the XorII methyltransferase gene and a vsr homolog from Xanthomonas oryzae pv. oryzae. Mol. Gen. Genet. 224, 383–390.
Choi, S. H., and Leach, J. E. (1994) Genetic manipulation of X. oryzae pv. oryzae. Int. Rice Res. Notes 19, 31,32.
Shaw, J. J., and Khan, I. (1993) Efficient transposon mutagenesis of Xanthomonas campestris pathovar campestris by high-voltage electroporation. BioTechniques 14, 556–558.
Wang, R. Y. H., Shedlarski, J. G., Farber, M. B., Kuebbing, D., and Ehrlich, M. (1980) Two sequence specific endonucleases from Xanthomonas oryzae. Biochim. Biophys. Acta. 606, 371–385.
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© 1995 Humana Press Inc.
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White, T.J., Gonzalez, C.F. (1995). Electroporation of Xanthomonas . In: Nickoloff, J.A. (eds) Electroporation Protocols for Microorganisms. Methods in Molecular Biology™, vol 47. Humana Press. https://doi.org/10.1385/0-89603-310-4:135
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DOI: https://doi.org/10.1385/0-89603-310-4:135
Publisher Name: Humana Press
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