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Studies of Protein-Ligand Interactions by NMR

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Protein NMR Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 60))

Abstract

NMR spectroscopy is recognized widely as an invaluable tool for the structural characterization of biological macromolecules with molecular weights of less than approx 25 kDa. The quality of structures obtainable using NMR spectroscopic methods is comparable with those derived from X-ray crystallography but, in addition, NMR offers the possibility of obtaining quantitative information on molecular flexibility. A particularly important aspect of the dynamics of macromolecules is that of ligand binding. Such binding can be accompanied by conformational changes in either the ligand, the macromolecule, or both and, in many cases, such dynamic changes are crucial to the functioning of the macromolecular system. This chapter is concerned with the use of NMR to define the nature of specific protein-ligand interactions. Although the focus is on interactions of ligands with proteins, rather than with other biological macromolecules, such as DNA or membranes, many of the techniques applicable to studies of protein-ligand interactions generally are applicable also to other macromolecular interactions. The topic of protein-ligand interactions has also been addressed from a number of different viewpoints in several other recent reviews (1–7).

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Author information

Authors and Affiliations

  1. Centre for Drug Design and Development, University of Queensland, St Lucia, Brisbane, Australia

    David J. Craik

  2. Department of Medicinal Chemistry, Victorian College of Pharmacy, Monash University, Victoria, Australia

    Jacqueline A. Wilce

Authors
  1. David J. Craik
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  2. Jacqueline A. Wilce
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Editor information

Editors and Affiliations

  1. SmithKline Beecham Pharmaceuticals, Welwyn, Hertfordshire, UK

    David G. Reid

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© 1997 Humana Press Inc.

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Craik, D.J., Wilce, J.A. (1997). Studies of Protein-Ligand Interactions by NMR. In: Reid, D.G. (eds) Protein NMR Techniques. Methods in Molecular Biology™, vol 60. Humana Press. https://doi.org/10.1385/0-89603-309-0:195

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  • DOI: https://doi.org/10.1385/0-89603-309-0:195

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-309-2

  • Online ISBN: 978-1-59259-546-4

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