Abstract
Various methods have been devised to detect antibody-producing cells in culture and also determine the isotype of the immunoglobulin produced. We have found the ELISPOT assay to be the method of choice for detecting human monoclonal antibodies (MAbs) produced by heterohybridomas (see Chapter 4) (1). The method used in our laboratory to detect and isotype human MAb specific to bacterial antigens is described here.
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Reference
Czerkinsky, C., Nilsson, L. A., Tarkowski, A., Koopman, W. J., Mestecky, J., and Ouchterlony, O. (1988). The enzyme-linked immunospot assay for detection of specific antibody-secreting cells: methodology and applicability, in Theoretical and Technical Aspects of ELISA and Other Solid Phase Immunoassays (Kemeny, D. M. and Challacombe, S. J., eds.) Wiley, Chichester, UK, pp. 217–239.
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© 1995 Humana Press Inc.
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Cooper, M.D., Kirkpatrick, R. (1995). Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay. In: Davis, W.C. (eds) Monoclonal Antibody Protocols. Methods in Molecular Biology™, vol 45. Humana Press. https://doi.org/10.1385/0-89603-308-2:83
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DOI: https://doi.org/10.1385/0-89603-308-2:83
Publisher Name: Humana Press
Print ISBN: 978-0-89603-308-5
Online ISBN: 978-1-59259-532-7
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