Abstract
The first step in preparing useful monoclonal antibodies (MAbs) is to immunize an animal with an appropriate “vaccine.” Animal and vaccine are both emphasized in the preceding sentence because this chapter describes how to generate satisfactory MAbs by maximizing interactions between the two. The term vaccine was used purposefully to connote that not only antigens of interest may be contained in the immunizing product, but carriers and adjuvants may also be included. These latter components can influence greatly the success of obtaining useful hybridomas, which produce antibodies of the desired specificity and quality.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Davis, B. D., Dulbecco, R., Eisen, H. N., Ginsberg, H. S., Wood, W. B., and McCarty, M. (1973) Antibody formation, in Microbiology, 2nd ed., Harper & Row, New York, pp. 484,485.
Benjamini, E. and Leskowitz, S. (1991) Immunology. A Short Course, 2nd ed., Wiley-Liss, New York, pp. 38–40.
Kabat, E. A. and Mayer, M. M. (1961) Experimental Immunochemistry, 2nd ed., Thomas, Springfield, IL, pp. 446–450, 798–802, 813–815.
Hui, G. S. N., Chang, S. P., Gibson, H., Hashimoto, A., Hashiro, C., Barr, P. J., and Kotani, S. (1991) Influence of adjuvants on the antibody specificity to the Plasmodium falciparum major merozoite surface protein, gp195. J. Immunol. 147, 3935–3941.
Kabat, E. A. and Mayer, M. M. (1961) Experimental Immunochemisty, 2nd ed., Thomas, Springfield, IL, pp. 309–310, 872.
Rudbach, J. A., Johnson, D. A., and Ulrich, J. T. (1995) Ribi adjuvants: chemistry, biology and utility in vaccines for human and veterinary medicine, in Adjuvants Theory and Practical Applications (Stewart-Tull, D. E. S., ed.), Wiley, New York, pp. 287–313.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Humana Press Inc.
About this protocol
Cite this protocol
Rudbach, J.A., Cantrell, J.L., Ulrich, J.T. (1995). Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies. In: Davis, W.C. (eds) Monoclonal Antibody Protocols. Methods in Molecular Biology™, vol 45. Humana Press. https://doi.org/10.1385/0-89603-308-2:1
Download citation
DOI: https://doi.org/10.1385/0-89603-308-2:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-308-5
Online ISBN: 978-1-59259-532-7
eBook Packages: Springer Protocols