Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies

  • Morris D. Cooper
  • Risa Kirkpatrick
Part of the Methods in Molecular Biology™ book series (MIMB, volume 45)

Abstract

In many circumstances, it is advantageous to have a continuous source of human antibody of a given specificity and immunoglobulin isotype. Reliance on human volunteers as a source of such antibody is problematic. Therefore, it has been a goal of investigators to establish immortal cell lines that produce the desired human antibody. The methods described in this chapter are a way to achieve this objective.

Keywords

Pyruvate Bicarbonate HEPES Myeloma Gentamicin 

References

  1. 1.
    Teng, N. N. H., Reyes, G. R., Bieber, M., Fry, K. E., Lam, K. S., and Herbert, J. M. (1985) Strategies for stable human monoclonal antibody production, in Human Hybridomas and Monoclonal Antibodies (Engleman, E. G., Foung, S. K. H., Larrick, J., and Raubitschek A., eds.), Plenum, New York, pp. 71–91.Google Scholar
  2. 2.
    Ostberg, L. and Pursch, E. (1983) Human × (mouse × human) hybridomas stably producing human antibodies. Hybridoma 2, 361–367.PubMedCrossRefGoogle Scholar
  3. 3.
    Kearney, J. F., Radbruch, A., Liesegang, B., and Rajewsky, K. (1979) A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123, 1548–1550.PubMedGoogle Scholar
  4. 4.
    Oi, V. T. and Herzenberg, L. A. (1980) Immunoglobulin-producing hybrid cell lines, in Selected Methods in Cellular Immunology (Mishell, B. and Shiigi, S. M., eds.), Freeman, San Francisco, pp. 351–372.Google Scholar
  5. 5.
    Bazin, R. and Lemieux, R. (1989) Increased proportion of B-cell hybridomas secreting monoclonal antibodies of desired specificity in cultures containing macrophage-derived hybridoma growth factor (IL6). J. Immunol. Methods 116, 245–249.PubMedCrossRefGoogle Scholar
  6. 6.
    Norden, R. P. and Potter, M. (1986) A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science 233, 566–569.CrossRefGoogle Scholar
  7. 7.
    Yokoyama, W. M. (1994) Preparation of cloning expansion medium, in Current Protocols in Immunology (Coligan, J. E., Kruisbeck, A. M., Margulies, D. H., Shevach, E. M., and Strober, W., eds.), Wiley, New York, pp. 2.5.11–2.5.12.Google Scholar

Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Morris D. Cooper
    • 1
  • Risa Kirkpatrick
    • 1
  1. 1.Department of Medical Microbiology and ImmunologySouthern Illinois University School of MedicineSpringfield

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