Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies

  • Morris D. Cooper
  • Risa Kirkpatrick
Part of the Methods in Molecular Biology™ book series (MIMB, volume 45)


In many circumstances, it is advantageous to have a continuous source of human antibody of a given specificity and immunoglobulin isotype. Reliance on human volunteers as a source of such antibody is problematic. Therefore, it has been a goal of investigators to establish immortal cell lines that produce the desired human antibody. The methods described in this chapter are a way to achieve this objective.


ELISPOT Assay Fusion Partner Conical Centrifuge Tube Immortal Cell Line Macroscopic Growth 
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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Morris D. Cooper
    • 1
  • Risa Kirkpatrick
    • 1
  1. 1.Department of Medical Microbiology and ImmunologySouthern Illinois University School of MedicineSpringfield

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