Abstract
The methods of induced cell fusion are very useful procedures in reproductive and developmental biology. They are used to answer basic questions associated with cell-cycle regulation in mammalian oocytes and embryos (1,2), to produce tetraploid embryos (3), and of particular interest, these procedures are central to the construction of clones, i.e., by allowing nuclear transplantation in mammals (see Chapter 26; 4,5). Various techniques can be used for the induction of fusion. The most commonly used in mammalian embryology are the techniques of Sendai virus-induced fusion, polyethylene glycol (PEG)-induced fusion, and the presently widely used technique of electrofusion (6,7).
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Fulka, J., Jr., and Fulka, J. (1991) Regulation of processes involved in mammalian oocyte maturation. Bull. Assoc. Anat. 228, 59–61.
Moor, R. M. (1988) Regulation of the meiotic cycle in oocytes of domestic animals. Ann. NY Acad. Sci. 541, 248–258.
Nagy, A., and Rossant, J. (1993) Production of completely ES-derived fetuses, in Gene Targeting, A Practical Approach (Joyner, A. L., ed.), IRL, London, pp. 147–179.
Robl, J. M., Collas, P., Fissore, R., and Dobrinsky, J. (1992) Electrically induced fusion and activation in nuclear transplant embryos, in Guide to Electroporation and Electrofusion (Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E., eds.), Academic, New York, pp. 535–551.
Smith, L. C. (1992) Production of genetically identical embryos by electrofusion, in Guide to Electroporation and Electrofusion (Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E., eds.), Academic, New York, pp. 371–391.
(1984) Cell Fusion, Ciba Foundation Symposium 103, Pitman, London, p. 282.
Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E., eds. (1992) Guide to Electroporation and Electrofusion. Academic, New York, p. 569.
Hogan, B., Constantini, F., and Lacy, E., eds. (1986) Manipulating the Mouse Embryo, A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 315.
Monk, M., ed. (1987) Mammalian Development, A Practical Approach. IRL, London, p. 306.
Dziuk, P., and M. Wheeler, W., eds. (1991) Handbook of Methods for the Study of Reproductive Physiology in Domestic Animals. University of Illinois Press.
Fulka, J., Jr. and Moor, R. M. (1993) Noninvasive chemical enucleation of mouse oocytes. Mol. Reprod. Dev. 34, 427–430.
Fulka, J., Jr., Notarianni, E., Passoni, L., and Moor, R. M. (1993) Early changes in embryonic nuclei fused to chemically enucleated mouse oocytes. Int. J. Dev. Biol. 7, 433–439.
Tarkowski, A. K. (1977) In vitro development of haploid mouse embryos produced by bisection of one-cell fertilised eggs. J. Embryol. Exp. Morphol. 38, 187–202.
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© 1995 Humana Press Inc.
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Fulka, J., Moor, R.M., Fulka, J. (1995). Electrofusion of Mammalian Oocytes and Embryonic Cells. In: Nickoloff, J.A. (eds) Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology, vol 48. Humana Press. https://doi.org/10.1385/0-89603-304-X:309
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DOI: https://doi.org/10.1385/0-89603-304-X:309
Publisher Name: Humana Press
Print ISBN: 978-0-89603-304-7
Online ISBN: 978-1-59259-535-8
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