Abstract
Electroporation is now a standard method of transfection and cell loading. There is a variety of commercial electroporation equipment, and many published and manufacturer-supplied protocols. Many of these protocols are results of trial and error. These empirical protocols are valuable guides for successful applications of electroporation.
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References
Abidor, I. G., Arakelyan, V. B., Chernomordik, L. V., Chizmadzhev, Y. A., Pastushenko, V. F., and Tarsevich, M. R. (1979) Electric breakdown of bilayer membranes. I. The main experimental facts and their qualitative discussion. Bioelectrochem. Bioenerg. 6, 37–52.
Zhelev, D. V., and Needham, D. (1993) Tension-stabilized pores in gaint vesicles: determination of pore size and pore line tension. Biochim. Biophys. Acta. 1147, 89–104.
Stenger, D. A., Kaler, K. V. I. S., and Hui, S. W. (1991) Dipole interaction in electrofusion. Contributions of membrane potential and effective dipole interaction pressures. Biophys. J. 59, 1074–1084.
Schwister, K., and Deuticke, B. (1985) Formation and properties of aqueous leaks induced in human erythrocytes by electrical breakdown. Biochim. Biophys. Acta. 816, 332–348.
Klenchin, V. A., Sukharev, S. I., Serov, S. M., Chernomordik, L. V., and Chidmadzhev, Y. A. (1991) Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis. Biophys. J. 60, 804–811.
Wolf, H., Rols, M. P., Boldt, E., Neumann, E., and Teissie, J. (1994) Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophys. J. 66, 524–531.
Andreason, G. L., and Evans, G. A. (1989) Optimization of electroporation for transfection of mammalian cells. Anal. Biochem. 180, 269–275.
Sukharev, S. I., Klenchin, V. A., Serov, S. M., Chernomordik, L. V., and Chizmadzhev, Y. A. (1992) Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores. Biophys. J. 63, 1320–1327.
Xie, T. D., Sun, L., and Tsong, T. Y. (1990) Study of mechanisms of electric field-induced DNA transfection. I. DNA entry by surface binding and diffusion through membrane pores. Biophys. J. 58, 13–19.
Nickoloff, J. A. and Reynolds, R. J. (1992) Electroporation-mediated gene transfer efficiency is reduced by linear plasmid carrier DNAs. Anal. Biochem. 205(2), 237–243.
Rols, M. P. and Teissie, J. (1990) Electropermeabilization of mammalian cells. Quantitative analysis of the phenomenon. Biophys. J. 58(5), 1089–1098.
Liang, H., Purucker, W. J., Stenger, D. A., Kubiniec, R. T., and Hui, S. W. (1988) Uptake of fluoroscence-labeled dextrans by 10T 1/2 fibroblasts following permeation by rectangular and exponential-decay electric field pulses. BioTechniques 6, 550–558.
Prausnitz, M. R., Milano, C. D., Gimm, J. A., Langer, R., and Weaver, J. C. (1994) Quantitative study of molecular transport due to electroporation: uptake of bovine serum albumin by erythrocyte ghosts. Biophys. J. 66, 1522–1530.
Xie, T. D., and Tsong, T. Y. (1992) Study of mechanisms of electric field-induced DNA transfection. III. Electric parameters and other conditions for effective transfection. Biophys. J. 63, 28–34.
Kubiniec, R. T., Liang, H., and Hui, S. W. (1990) Effects of pulse length and pulse strength on transfection by electroporation. BioTechniques 8, 1–3.
Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E. (1992) Guide for Electroporation and Electrofusion. Academic, San Diego.
Baker, P. F. and Knight, D. E. (1983) High-voltage techniques for gaming access to the interior of cells: application to the study of exocytosis and membrane turnover. Methods Enzymol. 98, 28–37.
Xie, T. D., and Tsong, T. Y. (1990) Study of mechanisms of electric field-induced DNA transfection. II. Transfection by low amplitude low frequency alternating electric fields. Biophys. J. 58, 897–903.
Chang, D. C., Gao, P. Q., and Maxwell, B. L. (1991) High efficiency gene transfection by electroporation using a radio-frequency electric field. Biochim. Biophys. Acta. 1992, 153–160.
Tekle, E., Austumian, R. D., and Chock, P. B. (1991) Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells. Proc. Natl. Acad. Sci. USA 88, 4230–4234.
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© 1995 Humana Press Inc.
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Hui, S.W. (1995). Effects of Pulse Length and Strength on Electroporation Efficiency. In: Nickoloff, J.A. (eds) Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology, vol 48. Humana Press. https://doi.org/10.1385/0-89603-304-X:29
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DOI: https://doi.org/10.1385/0-89603-304-X:29
Publisher Name: Humana Press
Print ISBN: 978-0-89603-304-7
Online ISBN: 978-1-59259-535-8
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