Spectrofluorometric Assay for Cell-Tissue Electrofusion

  • Richard Heller
Part of the Methods in Molecular Biology book series (MIMB, volume 48)


Electrofusion is a process by which fusion between cell membranes can be induced by exposure to electrical fields (1,2). Many practical applications of electrofusion have been demonstrated, such as the formation of hybridomas (3, 4, 5, 6), the production of monoclonal antibodies (MAb) (7, 8, 9), studying membrane fusion mechanisms (10, 11, 12, 13, 14), and examining cytosolic events (15, 16, 17). In addition, liposome-cell fusion has been utilized for the introduction of material into cells (18). Cell-tissue electrofusion (CTE) represents another electrofusion area of interest. In this process, individual cells are incorporated into intact tissue (19). CTE has been performed in vivo and has been shown to be useful for the interspecies transfer of membrane-surface components (20).


HL60 Cell Intact Tissue Rabbit Cornea Human HL60 Promyelocytic Leukemia Cell Human HL60 
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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Richard Heller
    • 1
    • 2
  1. 1.Department of Chemical Engineering, College of EngineeringUniversity of South FloridaTampa
  2. 2.Department of Surgery, College of MedicineTampa

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