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Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 44))

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Abstract

The commercial importance of oilseed rape (Brassica nupus L.) makes it desirable to have an efficient transformation protocol for practical applications. With the exception of a few reports, transformation experiments with B. napus using Agrobacterium tumefaciens yielding transgenic plants have been performed principally with the spring variety B. napus L. ssp. oleifera cv. Westar (1–5). Transformation protocols are relatively specific for cv. Westar and cannot be extended to most of the other spring and none of the winter varieties tested (6). There are only isolated reports on transgenic winter oilseed rape plants (6–8).

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Author information

Authors and Affiliations

  1. Agrochemicals Division, Research/Biotechnology, Pflanzenschutzzentrum Monheim, Bayer AG, Leverkusen (Bayerwerk), Germany

    Jürgen E. Thomzik

Authors
  1. Jürgen E. Thomzik
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Editor information

Editors and Affiliations

  1. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  2. Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

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© 1995 Humana Press Inc., Totowa, NJ

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Thomzik, J.E. (1995). Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.). In: Gartland, K.M.A., Davey, M.R. (eds) Agrobacterium Protocols. Methods in Molecular Biology™, vol 44. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-302-3:79

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  • DOI: https://doi.org/10.1385/0-89603-302-3:79

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-302-3

  • Online ISBN: 978-1-59259-531-0

  • eBook Packages: Springer Protocols

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