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T-DNA Transfer to Maize Plants

  • Wen-Hui Shen
  • Jesús Escudero
  • Barbara Hohn
Part of the Methods in Molecular Biology™ book series (MIMB, volume 44)

Abstract

Agrobacterium tumefaciens is a soil bacterium that induces, in most dicotyledonous plants, the neoplastic disease called crown gall. These tumors form at the site of wounding. The molecular basis for the tumor formation is the integration into and expression from the plant genome of T-DNA (transferred DNA), a part of the Ti (tumor-inducing) plasmid carried by the bacterium. T-DNA oncogenes specifying the synthesis of auxin and cytokinin are responsible for cell proliferation resulting in tumor growth. Additional T-DNA genes encode enzymes that produce, in the gall, novel amino acid and sugar derivatives, called opines, which are specific growth substrates for the bacterium. Different bacterial strains produce and consume different opines. Thus, strains have been classified accordingly as nopaline, octopine, or other types, depending on their diagnostic opine.

Keywords

Immature Embryo Agrobacterium Strain Maize Genotype Maize Streak Virus Maize Shoot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1995

Authors and Affiliations

  • Wen-Hui Shen
    • 1
  • Jesús Escudero
    • 2
  • Barbara Hohn
    • 2
  1. 1.Institut de Biologie Moléculaire des Plantes du CNRSUniversité Louis PasteurStrasbourgeFrance
  2. 2.Friedrich Miescher-InstitutBaselSwitzerland

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