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Agrobacterium tumefaciens Chemotaxis Protocols

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 44))

Abstract

Directed movement in response to chemical attractants is of crucial importance to Agrobacterium tumefaciens. Motility is a determinant of rhizosphere competence (1), and chemotaxis a conditional requirement for virulence (2,3). A. tumefaciens is attracted toward a variety of sugars typical of plant exudates (4). Responses to nanomolar amounts of phenolic wound exudates are chiefly determined by the Ti-plasmid encoded virulence genes virA and virG (5,6). The sensitivity of these responses indicates that they play a role in the biology of A. tumefaciens. Thus, the attraction to sugars can partly explain the organism’s prevalence in the rhizosphere (7) and the highly sensitive response to phenolics, may help to guide A. tumefaciens to wounded plant cells (8,9).

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References

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Durham, UK

    Charles H. Shaw

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  1. Charles H. Shaw
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Editor information

Editors and Affiliations

  1. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  2. Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

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© 1995 Humana Press Inc., Totowa, NJ

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Shaw, C.H. (1995). Agrobacterium tumefaciens Chemotaxis Protocols. In: Gartland, K.M.A., Davey, M.R. (eds) Agrobacterium Protocols. Methods in Molecular Biology™, vol 44. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-302-3:29

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  • DOI: https://doi.org/10.1385/0-89603-302-3:29

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-302-3

  • Online ISBN: 978-1-59259-531-0

  • eBook Packages: Springer Protocols

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