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Manipulating Photosynthesis in Transgenic Plants

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 44))

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Abstract

Agrobacterium tumefaciens-mediated transformation of plants provides a relatively straightforward means of altering the amounts of individual proteins involved in photosynthetic processes. By the introduction of additional copies of genes or of chimeric cDNA constructs, it is possible to produce transgenic plants with increased or decreased amounts of specific photosynthetic components. These plants can then be analyzed to examine the effects of the altered protein levels on a wide range of physiological or biochemical processes.

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References

  1. Last, D. I. and Gray, J. C. (1990) Synthesis and accumulation of pea plastocyanin in transgenic tobacco plants. Plant Mol. Biol. 14, 229–238.

    Article  PubMed  CAS  Google Scholar 

  2. Gray, J. C., Last, D. I., Dupree, P., Newman, B. J., and Slatter, R. E. (1990) Expression of genes for photosynthetic electron transfer components in transgenic plants, in Genetic Engineering of Crop Plants (Lycett, G. W. and Grierson, D., eds.), Butterworths, London, pp. 191–205.

    Google Scholar 

  3. Napoli, C., Lemieux, C., and Jorgensen, R. (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2, 279–289.

    Article  PubMed  CAS  Google Scholar 

  4. van der Krol, A. R., Mur, L. A., Beld, M., Mol, J. N. M., and Stuitje, A. R. (1990) Flavonoid genes in petunia addition of a limited number of gene copies may lead to suppression of gene expression. Plant Cell 2, 291–299.

    Article  PubMed  Google Scholar 

  5. van der Krol, A. R., Lenting, P. E., Veenstra, J., van der Meer, I. M., Koes, R. E., Gerats, A. G. M., et al. (1988) An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333, 866–869.

    Article  Google Scholar 

  6. Smith, C. J. S., Watson, C. F., Ray, J., Bird, C. R., Morris, P. C., Schuch, W., and Grierson, D. (1988) Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334, 724–726.

    Article  CAS  Google Scholar 

  7. Rodermel, S. R., Abbott, M. S., and Bogorad, L. (1988) Nuclear-organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants. Cell 55, 673–681.

    Article  PubMed  CAS  Google Scholar 

  8. Hudson, G. S., Evans, J. R., von Caemmerer, S., Arvidsson, Y. B. C., and Andrews, T. J. (1992) Reduction of ribulose-1, 5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol. 98, 294–302.

    Article  PubMed  CAS  Google Scholar 

  9. Stockhaus, J., Hofer, M., Renger, G., Westerhoff, P., Wydrzynski, T., and Willmitzer, L. (1990) Anti-sense RNA efficiently inhibits formation of the 10 kd polypeptide of photosystem II in transgenic potato plants: analysis of the role of the 10 kd protein. EMBO J. 9, 3013–3021.

    PubMed  CAS  Google Scholar 

  10. Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12, 8711–8721.

    Article  PubMed  CAS  Google Scholar 

  11. Odell, J. T., Nagy, F., and Chua, N.-H. (1985) Identification of DNA sequences required for the activity of cauliflower mosaic virus 35S promoter. Nature 313, 810–812.

    Article  PubMed  CAS  Google Scholar 

  12. Mazur, B. J. and Chui, C.-F. (1985) Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobacco. Nucleic Acids Res. 13, 2373–2386.

    Article  PubMed  CAS  Google Scholar 

  13. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  14. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680–685.

    Article  PubMed  CAS  Google Scholar 

  15. Shen, W. and Forde, B. G. (1989) Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucleic Acids Res. 17, 8385.

    Article  PubMed  CAS  Google Scholar 

  16. Horsch, R. B., Fry, J. E., Hoffmann, N., Eichhloltz, D., Rogers, S. G., and Fraley, R. T. (1985) A simple and general method for transferring genes to plants. Science 227, 1229–1231.

    Article  CAS  Google Scholar 

  17. Apel, K. and Kloppstech, K. (1978) The plastid membrane of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light harveting chlorophyll a/b protein. Eur. J. Biochem 85, 581–588.

    Article  PubMed  CAS  Google Scholar 

  18. Vaucheret, H., Kronenberger, J., Lepingle, A., Vilaine, F., Boutin, J.-P., and Caboche, M. (1992) Inhibition of tobacco nitrite reductase activity by expression of antisense RNA. Plant. J. 2, 559–569.

    PubMed  CAS  Google Scholar 

  19. Boutry, M. and Chua, N.-H. (1985) A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. EMBO J. 4, 2159–2165.

    PubMed  CAS  Google Scholar 

  20. van der Krol, A. R., Mur, L. A., de Lange, P., Mol, J. N. M., and Stuitje, A. R. (1990) Inhibition of flower pimentation by antisense CHS genes, promoter and minimal sequence requirements for antisense effect. Plant Mol. Biol. 14, 457–466.

    Article  PubMed  Google Scholar 

  21. Adamska, I., Scheel, B., and Kloppstech, K. (1991) Circadian oscillations of nuclear-encoded chloroplast proteins in pea (Pisum sativum). Plant Mol. Biol. 17, 1055–1065.

    Article  PubMed  CAS  Google Scholar 

  22. Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15.

    Article  PubMed  CAS  Google Scholar 

  23. Walker, D. A. (1987). The Use of the Oxygen Electrode and Fluorescence Probes in Simple Measurements of Photosynthesis. Oxygraphics Ltd., Sheffield, UK.

    Google Scholar 

  24. Walker, D. A. (1989) Automated measurement of leaf photosynthetic O2 evolution as a function of photon flux density. Phil. Trans. R. Soc. Lond. B 323, 313–326.

    Article  CAS  Google Scholar 

  25. Quick, W. P., Schurr, U., Scheibe, R., Schulze, E.-D., Rodermel, S. R., Bogorad, L., and Stitt, M. (1991) Decreased ribulose-1,5-bisphosphate carboxylase-oxygenase in transgenic tobacco transformed with “antisense” rbcS. I. Impact on photosynthesis in ambient growth conditions. Planta 183, 542–554.

    Article  CAS  Google Scholar 

  26. Stitt, M., Quick, W. P., Schurr, U., Schulze, E.-D., Rodermel, S. R., and Bogorad, L. (1991) Decreased ribulose-1,5-bisphosphate carboxylase-oxygenase in transgenic tobacco transformed with “antisense” rbcS. II. Flux-control coefficients for photosynthesis in varying light, CO2, and air humidity. Planta 183, 555–566.

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Plant Sciences, University of Cambridge, UK

    Jacqueline S. Knight, Francisco Madueño, Simon A. Barnes & John C. Gray

Authors
  1. Jacqueline S. Knight
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  2. Francisco Madueño
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  3. Simon A. Barnes
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  4. John C. Gray
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Editor information

Editors and Affiliations

  1. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  2. Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

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© 1995 Humana Press Inc., Totowa, NJ

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Knight, J.S., Madueño, F., Barnes, S.A., Gray, J.C. (1995). Manipulating Photosynthesis in Transgenic Plants. In: Gartland, K.M.A., Davey, M.R. (eds) Agrobacterium Protocols. Methods in Molecular Biology™, vol 44. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-302-3:263

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  • DOI: https://doi.org/10.1385/0-89603-302-3:263

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-302-3

  • Online ISBN: 978-1-59259-531-0

  • eBook Packages: Springer Protocols

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