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Histochemical GUS Analysis

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 44))

Abstract

Patterns in the localization of compounds, metabolic processes, and regulatory machineries at the cellular and subcellular levels are studied by scientific disciplines, called cytochemistry (biochemistry of the cell, cellular topochemistry) or histochemistry, if the tissue level is concerned. In order to reach these goals, a whole range of procedures have been developed, which may be divided in two groups: the in vitro techniques, based on homogenate fractionation and the in situ techniques, realized on sections. Only in situ techniques are considered in this chapter (1–5).

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Author information

Authors and Affiliations

  1. Faculty of Biology, University of South Bohemia, České Budějovice, Czech Republic

    Stanislav Vitha & Karel Beneš

  2. Gatersleben Research Institute, Gatersleben, Germany

    Julian P. Phillips

  3. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland

    Kevan M. A. Gartland

Authors
  1. Stanislav Vitha
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  2. Karel Beneš
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  3. Julian P. Phillips
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  4. Kevan M. A. Gartland
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Editor information

Editors and Affiliations

  1. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  2. Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

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© 1995 Humana Press Inc., Totowa, NJ

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Vitha, S., Beneš, K., Phillips, J.P., Gartland, K.M.A. (1995). Histochemical GUS Analysis. In: Gartland, K.M.A., Davey, M.R. (eds) Agrobacterium Protocols. Methods in Molecular Biology™, vol 44. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-302-3:185

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  • DOI: https://doi.org/10.1385/0-89603-302-3:185

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-302-3

  • Online ISBN: 978-1-59259-531-0

  • eBook Packages: Springer Protocols

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