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Agrobacterium Protocols pp 101–119Cite as

Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 44))

Abstract

The transfer of genetic material into soybean (Glycine max L. Merr.) plant tissues has been accomplished by several methods: electroporation (1), microprojectiles (2), and by the more widely used Agrobacterium-mediated T-DNA transfer (3,4). The transformation of soybean by electroporation-mediated gene transfer appears to be efficient for obtaining stable transformants in soybean cells (1). However, the disadvantage of this method is that the efficiency is affected by many variables, such as voltage, duration, and spacing of electrical pulses, size and number of protoplasts, buffer composition, temperature, concentration, and form of DNA. Thus, optimum electroporation must be determined for each protoplast type. The transformation of soybean by particle bombardment (2), in which the microprojectiles are used to transfer the genetic material into meristematic cells of immature seeds, appears to be simple and very effective. However, it requires a knowledge of the design of the acceleration instruments for those who wish to build their own, or at least access to an instrument that can control the acceleration of microprojectiles. These limitations presently restrict genetic engineering of soybean to some university and corporate laboratories.

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Authors and Affiliations

  1. Molecular Biology-Unit 7242, The Upjohn Company, Kalamazoo, MI

    Paula P. Chee & Jerry L. Slightom

Authors
  1. Paula P. Chee
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  2. Jerry L. Slightom
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Editor information

Editors and Affiliations

  1. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  2. Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

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© 1995 Humana Press Inc., Totowa, NJ

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Chee, P.P., Slightom, J.L. (1995). Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants. In: Gartland, K.M.A., Davey, M.R. (eds) Agrobacterium Protocols. Methods in Molecular Biology™, vol 44. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-302-3:101

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  • DOI: https://doi.org/10.1385/0-89603-302-3:101

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-302-3

  • Online ISBN: 978-1-59259-531-0

  • eBook Packages: Springer Protocols

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