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Measurement of Diacylglycerol

Origins of Receptor- Stimulated Diacylglycerol Accumulation
  • Michael R. Boarder
  • John R Purkiss
Part of the Methods in Molecular Biology™ book series (MIMB, volume 41)

Abstract

Diacylglycerol (DAG) found within cells is derived from two broad sources. First, in the de novo biosynthetic pathway, glycerol 3-phosphate is converted by two acylation steps to phosphatidic acid (PA), which is converted to DAG by phosphatidate phosphohydrolase (PPH). This is part of the pathway for synthesis of triacylglycerol and phospholipids from the carbon backbone of glycerol. Second, DAG is formed from phospholipids by the actions of phospholipases. It is this second process that is directly regulated by cell-surface receptors, and to which this chapter is addressed.

Keywords

Balance Salt Solution Phosphatidic Acid Phosphatidic Acid Adrenal Chromaffin Cell Inositol Phospholipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Lee, C. and Hajra, A. K (1991) Molecular species of diacylglycerols and phosphoglycendes and the postmortem changes in the molecular species of diacylglycerols in rat brains. J. Neurochem 56, 370–379.PubMedCrossRefGoogle Scholar
  2. 2.
    Holbrook, P. G., Pannell, L. K, Murata, Y., and Daly, J. W. (1992) Molecular species analysis of a product of phospholipase D activity. Phosphatidylethanol is formed from phosphatidylcholine in phorbol ester and bradykinin stimulated PC 12 cells. J. Biol. Chem. 267, 16,834–16,840.PubMedGoogle Scholar
  3. 3.
    Lee, C, Fisher, S. K., Agranoff, B. W., and Hajra A. K. (1991) Quantitative analysis of molecular species of diacylglycerol and phospatidate formed upon muscarinic receptor activation on human SK-N-SH neuroblastoma cells. J. Biol. Chem. 266, 22,837–22,846.PubMedGoogle Scholar
  4. 4.
    Pettitt, T. R. and Wakelam, M J O. (1993) Bombesin stimulates distinct time dependent changes in the sn-l,2-diradylglycerol molecular species profile from Swiss 3T3 fibroblasts as analysed by 3,5-dinitrobenzoyl denvatisation and h.p.l c. separation. Biochem J 289, 487–495.PubMedGoogle Scholar
  5. 5.
    Huang, C., Wykle, R. L., Daniel, L W., and Cabot, M C (1992) Identification of phosphatidylcholine-selective and phosphatidylinositol-selective phospholipases D in Madin-Darby canine kidney cells J. Biol. Chem 267, 16,859–16,865.PubMedGoogle Scholar
  6. 6.
    Loffelholz, K. (1989) Receptor regulation of choline phospholipid hydrolysis. Biochem. Pharmcol 38, 1543–1549CrossRefGoogle Scholar
  7. 7.
    Gumoz-Munoz, A., Hamza, E. H., and Brindley D. N (1992) Effects of sphingosine, albumin and unsaturated fatty acids on the activation and translocation of phosphatidate phosphohydrolase in rat hepatocytes. Biochim. Biophys. Acta 1127, 49–56Google Scholar
  8. 8.
    Martinson, E A, Trilivas, I., and Brown, J. H. (1990) Rapid protein kinase C dependent activation of phospholipase D leads to delayed 1,2-diglyceride accumulation J Biol Chem. 265, 22,282–22,287.PubMedGoogle Scholar
  9. 9.
    Kennedy, D. A. (1989) Diacylglycerol metabolism in mast cells. J Biol Chem. 264, 16,305–16,313Google Scholar
  10. 10.
    Preiss, J, Loomis, C. R., Bishop, W. R., Stein, R, Niedel, J. E., and Bell, R. M. (1986) Quantitative measurement of sn-l,2-diacylglycerols present in platelets, hepatocytes, and ras and sis transformed normal rat kidney cells. J. Biol. Chem. 261, 8597–8600.PubMedGoogle Scholar

Copyright information

© Humana Press Inc , Totowa, NJ 1995

Authors and Affiliations

  • Michael R. Boarder
    • 1
  • John R Purkiss
    • 1
  1. 1.Department of Cell Physiology and PharmacologyUniversity of LeicesterUK

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