Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor

  • Jeremy M. Henley
  • David M. Kirkham
Part of the Methods in Molecular Biology™ book series (MIMB, volume 41)

Abstract

Excitatory amino acid receptors are the predominant type of neurotransmitter receptor in the vertebrate central nervous system (CNS). They are membrane-spanning proteins that mediate the stimulatory actions of glutamate and possibly other related endogenous amino acids. Excitatory amino acid receptors are crucial for fast excitatory neurotransmission; they are believed to be involved in the neuropathology of ischemic damage; and they have been implicated in many disease states, including Alzheimer’s disease and epilepsy (1, 2).

Keywords

Cholesterol EDTA Chloroform Fractionation NMDA 

References

  1. 1.
    Barnes, J. M. and Henley, J. M. (1992) Molecular characteristics of excitatory amino acid receptors. Prog. Neurobiol. 39, 113–133.PubMedCrossRefGoogle Scholar
  2. 2.
    Choi, D. W. (1988) Glutamate neurotoxicity and diseases of the nervous system. Neuron 1, 623–634.PubMedCrossRefGoogle Scholar
  3. 3.
    Sommer, B. and Seeburg, P. H. (1992) Glutamate receptor channels—novel properties and new clones. Trends Pharmacol. Sci. 13, 291–296.PubMedCrossRefGoogle Scholar
  4. 4.
    Collingridge, G. L. and Lester, R. A. J. (1989) Excitatory amino acrd receptors in the vertebrate central nervous system. Pharmacol. Rev. 40, 143–208.Google Scholar
  5. 5.
    Gregor, P., Eshhar, N., Ortega, A., and Teichberg, V. I. (1988) Isolation, immunochemical characterisation and localisation of the kainate subclass of glutamate receptor from chick cerebellum. EMBO J. 7, 2673–2679.PubMedGoogle Scholar
  6. 6.
    Hampson, D. R. and Wenthold, R. J. (1988) A kainic acid receptor from frog brain purified using domoic acid affinity chromatography. J. Biol. Chem. 263, 2500–2505.PubMedGoogle Scholar
  7. 7.
    Hampson, D. R., Hme, D., and Wenthold, R. J. (1987) Solubilisation of kainic acid binding sites from rat brain. J. Neurochem. 49, 1209–1215.PubMedCrossRefGoogle Scholar
  8. 8.
    Henley, J. M. and Barnard, E. A. (1989) Solubilisation and characterisation of a putative quisqualate-type glutamate receptor from chick brain. J. Neurochem. 53, 140–148.PubMedCrossRefGoogle Scholar
  9. 9.
    Henley, J M. and Oswald, R. E. (1988) Solubilisation and characterisation of kainate binding sites from goldfish brain. Biochem. Biophys. Acta. 937, 102–111.Google Scholar
  10. 10.
    Henley, J. M. and Barnard, E. A. (1989) Kainate receptors in Xenopus central nervous system: solubilisation with n-octyl-ß-D-glucopyranoside. J. Neurochem. 52, 31–37.PubMedCrossRefGoogle Scholar
  11. 11.
    Henley, J. M., Ambrosml, A., Krogsgaard-Larsen, P., and Barnard, E. A. (1989) Evidence for a single glutamate receptor of the ionotropic kainate/quisqualate type. New Biologist. 1, 153–158PubMedGoogle Scholar
  12. 12.
    Henley, J. M., Ambrosini, A., Rodriguez-Ithurralde, D., Sudan, H., Brackley, P., Kerry, C., et al. (1992) Purified unitary kainate/ alpha-amino-3-hydroxy-5-methyl-isooxazole-propionate (AMPA) and kainate/AMPA/N-methyl-D-aspartate receptors with Interchangeable subumts. Proc. Natl. Acad. Sci. USA 89, 4806–4810.PubMedCrossRefGoogle Scholar
  13. 13.
    Barnard, E. A., Ambrosnn, A., Sudan, H., Prestipino, G., Lu, Q., Rodriguez-Ithurralde, D. (1991) Purification and properties of a functional unitary non-NMDA receptor from Xenopus brain, in Excitatory Amino Acids (Meldrum, B. S., Moroni, F., Simon, R. P., and Woods, J. H., eds ), Raven, New York, pp. 135–143.Google Scholar
  14. 14.
    Changeux, J. P. (1991) The mcotinic acetylcholine receptor: an allosteric protein prototype of ligand-gated ion channels. Trends Pharmacol. Sci 11, 485–491.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc , Totowa, NJ 1995

Authors and Affiliations

  • Jeremy M. Henley
    • 1
  • David M. Kirkham
    • 2
  1. 1.Department of Anatomy, School of Medical SciencesUniversity of BristolUK
  2. 2.Department of Pharmacology, Medical SchoolUniversity of BirminghamUK

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