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Plasmid Profile Typing and Plasmid Fingerprinting

  • Protocol
Diagnostic Bacteriology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 46))

Abstract

Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids range in size from less than one megaDalton (MDa) to several hundred MDa. One megaDalton of double-stranded DNA is equivalent to 1500 bp or 1.5 kilobases (kb, see Note 1).

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References

  1. Schmidt, T. J., Barret, J. S., Schrader, J. S., Scherach, C. S., McGee, H. P., Feldman, R. A., and Brenner D. J. (1982) Salmonellosis associated with marijuana. A multi-state outbreak traced by plasmid fingerprinting. N. Engl. J. Med. 306, 1249–1253.

    Article  PubMed  Google Scholar 

  2. Threlfall, E. J., Hall, M. L. M., and Rowe, B. (1986) Salmonella gold-coast from outbreaks of food-poisoning in the British Isles can be differentiated by plasmid profiles. J. Hyg. 97, 115–122.

    Article  CAS  Google Scholar 

  3. Threlfall, E. J., Hall, M. L. M., Ward, L. R., and Rowe, B. (1992) Plasmid profiles demonstrate that an upsurge in Salmonella berta in Humans in England and Wales is associated with imported poultry meat. Eur. J. Epidemiol. 8, 27–33.

    Article  PubMed  CAS  Google Scholar 

  4. Olsen, J. E., Brown, D. J., Baggesen, D. L., and Bisgaard, M. (1992) Biochemical and molecular characterization of Salmonella enterica serovar berta and comparison of methods for typing. Epidemiol. Infect. 108, 243–260.

    Article  PubMed  CAS  Google Scholar 

  5. Wray, C., Mclaren, I., Parkinson, N. M., and Beedell, Y. (1987) Differentiation of Salmonella typhimurium DT204c by plasmid profile and biotyping. Vet. Rec. 121, 514–516.

    Article  PubMed  CAS  Google Scholar 

  6. Brown, D. J., Baggesen, D. L., Hansen, H. B., Hansen, H. C., and Bisgaard, M. (1994) The characterization of Danish isolates of Salmonella enterica serovar Enteritidis by phage typing and plasmid profiling: 1980–1990. APMZS 102, 208–214.

    CAS  Google Scholar 

  7. Threlfall, E. J., Hampton, M. D., Chart, H., and Rowe, B. (1994) Use of plasmid profile typing for surveillance of Salmonella enteritidis phage type 4 from humans, poultry and eggs. Epidemiol. Infect. 112, 25–31.

    Article  PubMed  CAS  Google Scholar 

  8. Birnboim, H. C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic. Acids Res. 7, 1513–1523.

    Article  PubMed  CAS  Google Scholar 

  9. Kado, C. I. and Liu, S.-T. (1981) Rapid procedure for detection and isolation of large and small plasmids. J. Bact. 145, 1365–1373.

    PubMed  CAS  Google Scholar 

  10. Platt, D. J., Chesham, J. S., Brown, D. J., Kraft, C. A., and Taggart, J. (1986) Restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application. J. Hyg. 97, 205–210.

    Article  CAS  Google Scholar 

  11. Platt, D. J., Taggart, J., and Heraghty, K. A. (1988) Molecular divergence of the serotype-specific plasmid (pSLT) among strains of Salmonella typhimurium of human and veterinary origin and comparison of pSLT with the serotype specific plasmids of S. enteritidis and S. dublin. J. Med. Microbiol. 27, 277–284.

    Article  PubMed  CAS  Google Scholar 

  12. Brown, D. J., Threlfall, E. J., Hampton, M. D., and Rowe, B. (1993) Molecular characterization of plasmids in Salmonella enteritidis phage types. Epidemiol. Infect. 110, 209–216.

    Article  PubMed  CAS  Google Scholar 

  13. Bensaude, O. (1988) Ethidium bromide and safety. Trends Genet. 4, 89.

    Article  CAS  Google Scholar 

  14. Macrina, F. L., Kopecko, D. J., Jones, K. R., Ayers, D. J., and McCowen, S. M. (1978) A multiple plasmid-containing Escherichia coli: convenient source of plasmid size reference molecules. Plasmid 1, 417–420.

    Article  PubMed  CAS  Google Scholar 

  15. Threlfall, E. J., Rowe, B., Ferguson, J. L., and Ward, L. R. (1986) Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J. Hyg. 97, 419–426.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK

    E. John Threlfall & Neil Woodford

Authors
  1. E. John Threlfall
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  2. Neil Woodford
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Editor information

Editors and Affiliations

  1. Dalgety plc, Cambridge, UK

    Jenny Howard

  2. Department of Medicine, University of Cambridge, UK

    David M. Whitcombe

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© 1995 Humana Press Inc.

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Threlfall, E.J., Woodford, N. (1995). Plasmid Profile Typing and Plasmid Fingerprinting. In: Howard, J., Whitcombe, D.M. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 46. Humana Press. https://doi.org/10.1385/0-89603-297-3:225

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  • DOI: https://doi.org/10.1385/0-89603-297-3:225

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-297-2

  • Online ISBN: 978-1-59259-533-4

  • eBook Packages: Springer Protocols

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