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Pulsed Field Gel Electrophoresis

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Diagnostic Bacteriology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 46))

Abstract

The technique of pulsed field gel electrophoresis (PFGE) enables researchers to separate linear DNA fragments of up to 10 Mb. The technique was first described by Schwartz and Cantor (1) and differs from conventional gel electrophoresis by requiring the DNA molecules periodically to change their direction of migration.

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Author information

Authors and Affiliations

  1. Division of Food Science and Technology, Dairy Research Laboratory, CSIRO, Victoria, Australia

    Alan J. Hillier

  2. Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia

    Barrie E. Davidson

Authors
  1. Alan J. Hillier
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  2. Barrie E. Davidson
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Editor information

Editors and Affiliations

  1. Dalgety plc, Cambridge, UK

    Jenny Howard

  2. Department of Medicine, University of Cambridge, UK

    David M. Whitcombe

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© 1995 Humana Press Inc.

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Hillier, A.J., Davidson, B.E. (1995). Pulsed Field Gel Electrophoresis. In: Howard, J., Whitcombe, D.M. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 46. Humana Press. https://doi.org/10.1385/0-89603-297-3:149

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  • DOI: https://doi.org/10.1385/0-89603-297-3:149

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-297-2

  • Online ISBN: 978-1-59259-533-4

  • eBook Packages: Springer Protocols

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