Abstract
The contamination of food with organisms, such as Salmonella, Listeria, and Escherichia coli, continues to be an area of concern for consumers and food producers. Historically, the identification of such organisms has been done using conventional culture methods and biochemical techniques. These methods, although still considered by most to be the “gold standard” for microbial identification, can often be time consuming and laborious to perform. In addition, these methods can also be complicated by the heterogeneous nature of food microflora and the subjective nature of many microbiological and biochemical techniques.
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References
King, W., Raposa, S., Warshaw, J., Johnson, A., Halbert, D., and Klinger, J. D. (1989) A new colorimetric DNA hybridization assay for Listeria in foods. Int. J. Microbiol. 8, 225–232.
Fitts, R., Diamond, M., Hamilton, C., and Neri, M. (1983) DNA-DNA hybridization assay for detection of Salmonella spp. in foods. Appl. Environ. Microbiol. 46, 1146–1151.
Flowers, R. S., Klatt, M. J., Mozola, M. A., Curiale, M. S., Gabis, D. A., and Silliker, J. H. (1987) DNA hybridization assay for the detection of Salmonella in foods. J. Assoc. Off. Anal. Chem. 70, 521–529.
Hill, W. E. (1981) DNA hybridization method for detecting enteropathogenic Escherichia coli in human isolates and its possible application to food samples. J. Food Safety 3, 233–247.
Hsu, H. Y., Chan, S. W., Sobel, D. I., Halbert, D. N., and Groody, E. P. (1991) A calorimetric DNA hybridization method for the detection of Escherichia coli in foods. J. Food Prot. 54, 249–255.
Wilson, S. G., Chan, S., Deroo, M., Vera-Garcia, M., Johnson, A., Lane, D., and Halbert, D. N. (1990) Development of a calorimetric second generation nucleic acid hybridization method for the detection of Salmonella in foods and a comparison with conventional culture procedure. J. Food Prot. 55, 1394–1398.
U. S. Food and Drug Administration (1991) Bacteriological Manual, 7th ed. Association of Official Analytical Chemists, Arlington, VA.
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© 1995 Humana Press Inc.
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Groody, E.P. (1995). Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format. In: Howard, J., Whitcombe, D.M. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 46. Humana Press. https://doi.org/10.1385/0-89603-297-3:133
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DOI: https://doi.org/10.1385/0-89603-297-3:133
Publisher Name: Humana Press
Print ISBN: 978-0-89603-297-2
Online ISBN: 978-1-59259-533-4
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