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The Direct Epifluorescent Filter Technique (DEFT)

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Diagnostic Bacteriology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 46))

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Abstract

Food, water, pharmaceutical, and medical microbiology ideally need methods of detecting, identifying, and quantifying microorganisms that can give results within a few hours. The many days needed for conventional enrichment, plating, and biochemical/serological methods to give results do not enable problems to be identified soon enough to allow the appropriate remedial action to be implemented. New methods must produce rapid, accurate, and reliable estimates of total viable microbial numbers, spoilage, or indicator organisms or to identify the presence of pathogens.

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References

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Author information

Authors and Affiliations

  1. Torry Research Station, Aberdeen, UK

    Rohan Kroll

Authors
  1. Rohan Kroll
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Editor information

Editors and Affiliations

  1. Dalgety plc, Cambridge, UK

    Jenny Howard

  2. Department of Medicine, University of Cambridge, UK

    David M. Whitcombe

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© 1995 Humana Press Inc.

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Kroll, R. (1995). The Direct Epifluorescent Filter Technique (DEFT). In: Howard, J., Whitcombe, D.M. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 46. Humana Press. https://doi.org/10.1385/0-89603-297-3:113

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  • DOI: https://doi.org/10.1385/0-89603-297-3:113

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-297-2

  • Online ISBN: 978-1-59259-533-4

  • eBook Packages: Springer Protocols

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