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Preparation of Nonradioactive DNA Probes

  • Adrian Eley
  • Kevin M. Oxley
Part of the Methods in Molecular Biology™ book series (MIMB, volume 46)

Abstract

This chapter describes a simple method of making DNA probes with a nonisotopic label. The development of this type of new technology has, for example, created novel ways of detecting and identifying pathogenic microorganisms, which has led to a re-evaluation of methodological approaches in the diagnostic microbiology laboratory. At the same time, the move away from radioisotopes with all associated safety hazards has allowed DNA probe technology in general to become more accessible and more user friendly.

Keywords

Wash Buffer Chlamydia Trachomatis Film Cassette Fresh Container Secondary Wash Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Birnboim, H. C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513–1523.PubMedCrossRefGoogle Scholar
  2. 2.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Quantitation of DNA and RNA, in Molecular Cloning—A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. E5.Google Scholar

Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Adrian Eley
    • 1
  • Kevin M. Oxley
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of Sheffield Medical SchoolSheffield, South YorkshireUK

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