Abstract
Isolated plant protoplasts are most commonly liberated from tissues,
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Power, I. B and Davey, M. (1990) Protoplasts of higher and lower plants, in Methods in Molecular Biology 6. Plant Cell and Tissue Culture (Pollard, J. W. and Walker, J. M., eds.), Humana Press, Clifton, NJ, pp. 237–259.
Cocking, E. C. (1972) Plant cell protoplasts—solation and development. Annu. Rev. Plant. Physiol. 23, 29–50.
Tomes, D. T. (1990) Current research in biotechnology with applications to plant breeding, in Progress in Plant Cellular and Molecular Biology (Nijkamp, H J. J., Van Der Plas, L. H W, and Van Aartrijk, J., eds ), Kluwar, The Netherlands, pp 23–32.
John, M. E. and Stewart, J., McD. (1992) Genes for jeans: biotechnological advances in cotton. Trends Biotechnol. 10, 165–169
Pollard, J. W. and Walker, J. M. (eds.) (1990) Methods in Molecular Biology 6: Plant Cell and Tissue Culture Humana Press, Clifton, NJ.
Steponkus, P. L. (1985) Cryobiology of isolated protoplasts⊕pplications to plant cell cryopreservation, in Cryopreservation of Plant Cells and Organs (Kartha, K., ed.), CRC, Boca Raton, FL, pp. 49–60.
Steponkus, P. L. and Lynch, D. V. (1989) Freeze/thaw induced destabilisation of the plasma membrane and the effect of cold acclimation. J. Bioenerg. Biomembr. 21, 21–41.
Langis, R. and Steponkus, P L. (1991) Vitrification of isolated rye protoplasts: protectton against dehydration injury by ethylene glycol. Cryo-Lett. 12, 107–112.
Steponkus, P. L. and Weist, S C (1979) Freeze-thaw induced lesions in the plasma membrane in low temperature stress, in Crop Plants The Role of the Membrane (Lyons, J. M., Douglas, G., and Ratson, J. K., eds.), Academic, London, pp. 231–254
Kartha, K. (ed.) (1985) Cryopreserwtion of Plant Cells and Organs. CRC, Boca Raton, FL.
Withers, L. A. (1987) The low temperature preservation of plant cell, tissue and organ cultures and seed for genetic conservation and improved agricultural practice, in The Effects of Low Temperatures on Biological Systems (Grout, B. W. W and Morris, G. J, eds.), Edward Arnold, London, pp. 389–409.
Withers, L A. (1990) Cryopreservation of plant cells, in Methods in Molecular Biology 6: Plant Cell and Tissue Culture (Pollard, J. W. and Walker, J. M., eds.), Humana Press, Clifton, NJ, pp. 39–48.
Grout, B. W. W. (1990) Genetic preservation in vitro, in Progress in Plant Cellular and Molecular Biology (Nijkamp, H. J. J., Van Der Plas, L. H. W., and Van Aartrijk, J., eds.), Kluwar, The Netherlands, pp. 13–22.
Grout, B. W. W. (1991) Cryopreservation of plant cells and organs, in Conservation of Plant Genetic Resources Through In Vitro Methods (Zakri, A. H., Normah, M. N., Senawi, M. T., and Abdul Karim, A. G., eds), FRIM/MNCPGR, Kuala Lumpur, Malaysia, pp. 43–56.
Bajaj, Y. P. S. (1989) Cryopreservation of plant protoplasts, in Biotechnology in Agriculture and Forestry 8: Plant Protoplasts and Genetic Engineering (Bajaj, Y. P. S., ed.), Springer Verlag, Berlin, pp. 97–106.
Grout, B. W. W. and Morris, G. J (1987) Freezing and cellular organisation, in The Effects of Low Temperatures on Biological Systems (Grout, B. W. W. and Morris, G. J., eds.), Edward Arnold, London, pp. 147–174.
Grout, B. W. W. (1991) The effects of ice formation during cryopreservation of clinical systems, in Clinical Applications of Cryobiology (Fuller, B. J. and Grout, B. W. W., eds.), CRC, Boca Raton, FL, pp. 81–94
Fahy, G. M., MacFarlane, D. R., Angell, C. A. and Meryman, H. T. (1984) Vitrification as an approach to cryopreservation. Cryobiology 21, 407–426.
Franks, F. (1985) Biophysics and Biochemistry at Low Temperatures. Cambridge University Press, Cambridge, UK.
Taylor, M. J. (1987) Physico-chemical principles in low temperature biology, in The Effects of Low Temperatures on Biological Systems (Grout, B. W. W. and Morris, G. J., eds.), Edward Arnold, London, pp. 3–71.
Langis, R. and Steponkus, P. L. (1990) Cryopreservation of rye protoplasts by vitrification. Plant Physiol. 92, 666–671.
Langis, R. and Steponkus, P. L. (1991) Vitrification of isolated rye protoplasts: protection against dehydration injury by ethylene glycol. Cryo-Lett. 12, 107–112.
Takeuchi, M., Matushima, H., and Sugawara, Y. (1982) Totipotency and viability of protoplasts after long-term freeze preservation, in Plant Tissue Culture, 1982 (Fujiwara, A., ed.), Japanese Association for Plant Tissue Culture, Tokyo, pp 797,798.
Withers, L. A. (1985) Cryopreservation of cultured plant cells and protoplasts, in Cryopreservation of Plant Cells and Organs (Kartha, K., ed.), CRC, Boca Raton, FL, pp. 243–267.
Bajaj, Y. P. S. (1988) Regeneration of plants from frozen (−196°C) protoplasts of Atropa belladona L., Datum innoxia Mill., and Nicotiana tabacum L. Ind. J. Exp. Biol. 26, 289–292.
Gazeau, C. M., Hansz, J., Jondet, M., and Dereuddre, J. (1992) Cryomicroscopic comparison of freezing (to −40°C) and thawing effects on Catharunthus cells and their protoplasts. Cryo-Lett. 13, 137–148.
Gazeau, C. M., Blanchon, C., and Dereuddre, J. (1992) Freeze-preservation of Cutharunthus protoplasts at liquid nitrogen temperature. Comparison with cells. Cryo-Lett. 13, 149–158.
Withers, L. A. and King, P. J. (1980) A simple freezing unit and cryopreservatronmethod for plant cell suspensions. Cryo-Lett. 1, 213–220.
Sugawara, Y. and Steponkus, P. L. (1990) Effect of cold acclimation and modification of the plasma membrane lipid composition on lamellar to hexagonal phase transitions in rye protoplasts. Cryobiology 27, 667.
Webb, M. S. and Steponkus, P. L. (1990) Dehydration-induced hexagonal phase formation in phospholipid bilayers. Cryobiology 27, 666, 667.
Koster, K. L., Steponkus, P. L., and Lynch, V. (1989) Solute accumulation during cold acclimation of rye. Plant. Physiol. 89, S–26.
Pritchard, H. W., Grout, B. W. W., and Short, K. C. (1986) Osmotic stress as a pregrowth procedure for cryopreservation 3. Cryobiology of sycamore and soybean cell suspensions. Ann. Bot. 57, 379–388.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Humana Press Inc.
About this protocol
Cite this protocol
W., B. (1995). Cryopreservation of Plant Protoplasts. In: Day, J.G., Pennington, M.W. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology™, vol 38. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-296-5:91
Download citation
DOI: https://doi.org/10.1385/0-89603-296-5:91
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-296-5
Online ISBN: 978-1-59259-525-9
eBook Packages: Springer Protocols