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Cryopreservation of Fish Spermatozoa

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Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 38))

Abstract

Cryopreservation of fish spermatozoa has closely shadowed historical developments of the preservation technology used for mammalian gametes. Within 4 yr of the discovery of the cryoprotective value of glycerol (1), slices of Atlantic herring (Clupea harengus) testis were successfully cryopreserved and stored in dry ice for 6 mo in 80% (v/v) seawater containing 12.5% (v/v) glycerol (2).

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Author information

Authors and Affiliations

  1. Institute of Aquaculture, University of Stirling, Stirling, UK

    Krishen J. Rana

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  1. Krishen J. Rana
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Editor information

Editors and Affiliations

  1. The Windermere Laboratory, Institute of Freshwater Ecology, Cumbria, UK

    John G. Day

  2. Acer Environmental, Cheshire, UK

    Michael W. Pennington

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© 1995 Humana Press Inc.

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Rana, K.J. (1995). Cryopreservation of Fish Spermatozoa. In: Day, J.G., Pennington, M.W. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology™, vol 38. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-296-5:151

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  • DOI: https://doi.org/10.1385/0-89603-296-5:151

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-296-5

  • Online ISBN: 978-1-59259-525-9

  • eBook Packages: Springer Protocols

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