Abstract
Use of scanning electron microscopy (SEM) to study chromosomes permits their observation at higher resolution than is possible by light microscopy and, at the same time, provides aesthetically pleasing images. Chromosome preparations made for SEM in the three different ways described in this chapter are illustrated in Fig. 1–3. As can be seen, there is a general resemblance among chromosomes prepared by the three different techniques, but also some differences in detailed structure. At present, it is not possible to say which, if any, bears the closest resemblance to the in vivo state.
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References
Harrison, C J., Britch, M., Allen, T. D., and Harris, R (1981) Scanning electron microscopy of the G-banded human karyotype. Exp. CellRes. 134, 141–153
Mullinger, A. M. and Johnson, R. T. (1987) Disassembly of the mammalian metaphase chromosome into its subunits: studies with ultraviolet light and repair synthesis inhibitors. J. Cell Sci. 87, 55–69.
Sumner, A. T. (1991) Scanning electron microscopy of mammalian chromo somes from prophase to telophase. Chromosoma 100, 410–418.
Harrison, C. J, Jack, E M, and Allen, T. D., (1987) Light and scanning electron microscopy of the same metaphase chromosomes, in Correlative Microscopy in Biology. Instrumentation and Methods (Hayat, M A, ed.) Academic, New York, pp. 189–248.
Harrison, C J, Jack, E. M, Allen, T. D., and Haris, R. (1983) The fragile X-a scanning electron microscope study. J Med Genet 20, 280–285
Harrison, C J, Jack, E M, Allen, T D., and Harris, R (1985) Investigation of human chromosome polymorphisms by scanning electron microscopy. J. Med Genet 22, 16–23
Sumner, A. T. (1986) Electron microscopy of the parameres formed by the centromeric heterochromatin of human chromosome 9 at pachytene. Chromosoma, 94, 199–204
Wanner, G., Formanek, H., Martin, R., and Herrmann, R. G (1991) High resolution scanning electron microscopy of plant chromosomes. Chromosoma, 100, 103–109.
Stenman, S, Rosenqvist, M, and Ringertz, N R (1975) Preparation and spread of unfixed metaphase chromosomes for immunofluorescence staining of nuclear antigens. Exp. Cell Res. 90, 87–94.
Sillar, R. and Young, B. D. (1981) A new method for the preparation of metaphase chromosomes for flow analysis. J. Histochem. Cytochem. 29, 74–78
Perry, P E and Thomson, E J (1986) Immunogold labelling of metaphase cells Cytogenet. Cell Genet. 41, 121–125.
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© 1994 Humana Press Inc, Totowa, NJ
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Sumner, A.T., Ross, A.R., Graham, E. (1994). Preparation of Chromosomes for Scanning Electron Microscopy. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:41
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DOI: https://doi.org/10.1385/0-89603-289-2:41
Publisher Name: Humana Press
Print ISBN: 978-0-89603-289-7
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