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Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines

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Chromosome Analysis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 29))

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Abstract

The Yeast Artificial Chromosome (YAC) (1) technology allows the cloning of large fragments of DNA (several hundreds of kilobase pairs, kbp) into the budding yeast Saccharomyces cerevisiae, and therefore bridges a gap between conventional cloning in bacteria (λ and cosmid cloning) and somatic cell hybridization techniques. This powerful cloning system has been used successfully for the construction of representative human (2–5), mouse (5–5), Drosophila (8), and Caenorrhabditis elegans (9) genomic libraries.

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References

  1. Burke, D T, Carle, G F., and Olson, M. V (1987) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236, 806–812.

    Article  PubMed  CAS  Google Scholar 

  2. Brownstein, B H, Silverman, G A, Little, R. D., Burke, D. T, Korsmeyer, S. J, Schlessinger, D, and Olson, M. V. (1989) Isolation of single-copy human genes from a library of yeast artificial chromosome clones Science 244, 1348–1351.

    Article  PubMed  CAS  Google Scholar 

  3. Anand, R., Riley, J. H, Butler, R., Smith, J. C, and Markham, A. F. (1990) A 3 5 genome equivalent multi access YAC library construction, characterisation, screening and storage Nucleic Acids Res 18, 1951–1955.

    Article  PubMed  CAS  Google Scholar 

  4. Albertsen, H M., Abderrahim, H, Cann, H. M, Dausset, J., Le Paslier, D, and Cohen, D. (1990) Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. Proc. Natl. Acad Sci. USA 87, 4256–4260.

    Article  PubMed  CAS  Google Scholar 

  5. Larin, Z., Monaco, A P., and Lehrach, H. (1991) Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc. Natl. Acad. Sci USA 88, 4123–4127

    Article  PubMed  CAS  Google Scholar 

  6. Burke, D T, Rossi, J M., Leung, J., Koos, D S., and Tilghman, S. M (1991) A mouse library of yeast artificial chromosome clones Mammalian Genome 1, 65.

    Article  PubMed  CAS  Google Scholar 

  7. MacMurray, A. J., Weaver, A., Shin, H.-S, and Lander, E S. (1991) An automated method for DNA preparation from thousands of YAC clones Nucleic Acids Res. 19, 385–390.

    Article  PubMed  CAS  Google Scholar 

  8. Garza, D, Ajioka, J. W, Burke, D. T., and Hartl, D. L (1989) Mapping the Drosophila genome with yeast artificial chromosomes Science 246, 641–646.

    Article  PubMed  CAS  Google Scholar 

  9. Coulson, A., Waterson, R, Kiff, J, Sulston, J., and Kohara, Y. (1988) Genome linking with yeast artificial chromosomes. Nature 335, 184–186.

    Article  PubMed  CAS  Google Scholar 

  10. Coulson, A., Sulston, J, Brenner, S., and Karn, J. (1986) Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl. Acad Sci USA 83, 7821–7825

    Article  PubMed  CAS  Google Scholar 

  11. Elvin, P., Slynn, G., Black, D., Graham, A., Butler, R., Riley, J., Anand, R., and Markham, A F (1991) Isolation of cDNA clones using yeast artificial chromosome probes. Nucleic Acids Res 18, 3913–3917

    Article  Google Scholar 

  12. Kinzler, K W., Nilbert, M C, Su, L-K., Vogelstem, B, Bryan, T. M., Levy, D B., Smith, K J., Preisinger, A C, Hedge, P., McKechnie, D., Finniear, R., Markham, A, Groffen, J, Boguski, M. S., Altschul, S. F., Horii, A., Ando, H., Myioshi, Y, Miki, Y., Nishisho, I, and Nakamura, Y (1991) Identification of FAP locus genes from chromosome 5q21 Science 253, 661–665

    Article  PubMed  CAS  Google Scholar 

  13. Brookes, A J and Porteous, D. J (1991) Coincidence sequence cloning. Nucleic Acids Res. 19, 2609–2613.

    PubMed  CAS  Google Scholar 

  14. Pavan, W J., Hieter, P, and Reeves, R. J. (1990) Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes Proc. Natl Acad. Sci. USA 87, 1300–1304.

    Article  PubMed  CAS  Google Scholar 

  15. Campbell, C, Gulati, R, Nandi, A K., Floy, K., Hieter, P., and Kucherlapati, R. S. (1991) Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA. Proc. Natl. Acad. Sci USA 88, 5744–5748.

    Article  PubMed  CAS  Google Scholar 

  16. Green, E. D and Olson M V. (1990) Chromosomal region of the Cystic Fibrosis gene in yeast artificial chromosomes’ a model for human genome mapping. Science 250, 94–98.

    Article  PubMed  CAS  Google Scholar 

  17. Silverman, G. A., Green, E. D, Young, R. L, Jockel, J I, Domer, P. H., and Korsmeyer, S. J (1990) Meiotic recombination between yeast artificial chromosomes yields a single clone containing the entire BCL2 gene. Proc. Natl. Acad. Sci USA 87, 9913–9917.

    Article  PubMed  CAS  Google Scholar 

  18. Pachnis, V., Pevny, L., Rothstein, R, and Costantini, F. (1990) Transfer of a yeast artificial chromosome carrying human DNA from Saccharomyces cerevisiae into mammalian cells. Proc. Natl. Acad Sci. USA 87, 5109–5113.

    Article  PubMed  CAS  Google Scholar 

  19. Pavan, W J, Hieter, P., and Reeves, R. H. (1990) Modification and transfer into an embryonal carcinoma cell line of a 360-kilobase human-derived yeast artificial chromosome. Mol Cell. Biol 10, 4163–4169

    PubMed  CAS  Google Scholar 

  20. Fernandez-Luna, J. L., Matthews, R. J., Brownstein, B. H., Schreiber, R. D., and Thomas, M L. (1991) Characterization and expression of the human leukocyte-common antigen (CD45) gene contained in yeast artificial chromosomes. Genomics 10, 756–764

    Article  PubMed  CAS  Google Scholar 

  21. Jacobovits, A, Moore, A L, Green, L. L., Vergara, G J, Maynard-Cume, C. E., Austin, H. A., and Klapholtz, S. (1993) Germ-line transmission and expression of a human-derived yeast artificial chromosome Nature 362, 255–258.

    Article  Google Scholar 

  22. Schedl, A., Montoliu, L., Kelsey, G., and Schutz, G (1993) A yeast artificial chromosome covering the tyrosinase gene confers number-dependent expression in transgenic mice. Nature 362, 258–261.

    Article  PubMed  CAS  Google Scholar 

  23. Arveiler, B. and Porteous, D. J. (1992) Distribution of Alu and LI repeats in human YAC recombinants Mammalian Genome 3, 661–668.

    Article  PubMed  CAS  Google Scholar 

  24. Green, E. D, Riethman, H. C, Dutchik, J. E., and Olson, M V. (1991) Detection and characterization of chimeric yeast artificial chromosome clones. Genomics 11, 658–669

    Article  PubMed  CAS  Google Scholar 

  25. Bonetta, L, Kuehn, S. E., Huang, A., Law, D. J., Kalikin, L. M, Koi, M., Reeve, A. E., Brownstein, B. H., Yeger, H., Williams, B. R. G., and Feinberg, A. P. (1990) Wilms Tumor Locus on llpl3 defined by multiple CpG island-associated transcripts Science 250, 994–997

    Article  PubMed  CAS  Google Scholar 

  26. Silverman, G. A, Jockel, J. I, Domer, P. H., Mohr, R M, Taillon-Miller, P., and Korsmeyer, S J. (1991) Yeast artificial chromosome cloning of a two-megabase-size contig within chromosomal band 18q21 establishes physical linkage between BCL2 and Plasminogen Activator Inhibitor Type-2. Genomics 9, 219–228.

    Article  PubMed  CAS  Google Scholar 

  27. Arveiler, B., Murray, I., Stevenson, B., and Porteous, D. J. (1991) Construction of a library enriched for human chromosome 11 and Xp YAC recombinants. Mammalian Genome 1, 265–266.

    Article  PubMed  CAS  Google Scholar 

  28. Breen, M., Arveiler, B., Murray, I, Gosden, J. R., and Porteous, D. J. (1992) YAC mapping by FISH using Alu-PCR generated probes. Genomics 13, 726–730.

    Article  PubMed  CAS  Google Scholar 

  29. van Ommen, G. J. B. and Verkerk, J. M. H. (1986) Restriction analysis of chromosomal DNA in a size range up to two million base pairs by pulsed field gradient electrophoresis, in Analysis of Human Genetic Diseases (Davies, K., ed) IRL, Oxford, pp. 113–133

    Google Scholar 

  30. Maule, J. C. and Green, D K. (1990) Semi-conductor controlled contour-clamped homogeneous electric field apparatus. Anal. Biochem. 191, 390–395

    Article  PubMed  CAS  Google Scholar 

  31. Anand, R., Villasante, A., and Tyler-Smith, C. (1989) Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis Nucleic Acids Res. 17, 3425–3433

    Article  PubMed  CAS  Google Scholar 

  32. Chu, G., Vollrath, D., and Davis, R. W. (1986) Separation of large DNA molecules by contour-clamped homogeneous electric fields Science 234, 1582–1585.

    Article  PubMed  CAS  Google Scholar 

  33. Burgers, P. M. and Percival, K. J. (1986) Transformation of yeast spheroplastswithout cell fusion. Anal. Biochem. 163, 390–395.

    Google Scholar 

  34. McCormick, M K., Shero, J. H., Connelly, C J, Antonorakis, S. E., and Hieter, P. (1990) Methods for cloning large DNA segments as artificial chromosomes in S. cerevisiae. Technique 2, 65–71.

    CAS  Google Scholar 

  35. Saiki, R. K., Gelfand, D. H, Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Ehrlich, H. A. (1988) Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239, 487–491.

    Article  PubMed  CAS  Google Scholar 

  36. Green, E. D. and Olson, M V (1990) Systematic screening of yeast artificial chromosome libraries by use of polymerase chain reaction Proc. Natl Acad. Sci. USA 87, 1213–1217.

    Article  CAS  Google Scholar 

  37. Scherman, F, Fink, G R, and Hicks, J. B. (1983) Methods in Yeast Genetics Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  38. Feinberg, A. P. and Vogelstein, B. (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137, 266–267

    Article  PubMed  CAS  Google Scholar 

  39. Albertsen, H. M, Le Paslier, D, Abderrahim, H., Dausset, J., Cann, H., and Cohen, D (1989) Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg++ Nucleic Acids Res. 17, 808.

    Article  PubMed  CAS  Google Scholar 

  40. Arveiler, B., Vincent, A., and Mandel, J.-L. (1989) Toward a physical map of the Xq28 region in man: linking color vision, G6PD, and coagulation Factor VIII genes to an X-Y homology region. Genomics 4, 460–471.

    Article  PubMed  CAS  Google Scholar 

  41. Allshire, R. C (1990) Introduction of large linear minichromosomes into Schizosaccharomycespom.be by an improved transformation procedure. Proc. Natl. Acad Sci USA 87, 4043–4047

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department Biochimie Medicale, Universite de Bordeaux II, Bourdeaux Cédex, France

    BenoÎt Arveiler

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  1. BenoÎt Arveiler
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Editors and Affiliations

  1. Medical Research Council, Edinburgh, Scotland

    John R. Gosden

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© 1994 Humana Press Inc, Totowa, NJ

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Arveiler, B. (1994). Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:379

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  • DOI: https://doi.org/10.1385/0-89603-289-2:379

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-289-7

  • Online ISBN: 978-1-59259-516-7

  • eBook Packages: Springer Protocols

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