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Protocols for Chromosome-Mediated Gene Transfer

Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping

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Chromosome Analysis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 29))

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Abstract

Linkage studies in humans are strictly limited by such factors as family structure, the availability of material for study, and informative markers for linkage analysis. Parasexual approaches therefore have a particularly important role to play in human genetics (1). A panoply of somatic cell genetic methods have been devised and exploited. These include (1) immortalizing experimental and clinical mutations and chromosome aberrations as lymphoblastoid cell lines (Chapter 5 and 17) and (2) segregating individual chromosome or subchromosome fragments one from another by cell fusion or gene transfer for (a) physical mapping of existing markers, and (b) as the starting material for enrichment cloning by one technique or another. I will focus here on the technique of chromosome-mediated gene transfer, a somatic cell genetic method that can bridge the gap between the functional and molecular analysis of single genes or whole chromosomes.

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Author information

Authors and Affiliations

  1. Medical Research Council, Human Genetics Unit, Western General Hospital, Edinburgh, UK

    David J. Porteous

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  1. David J. Porteous
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Editors and Affiliations

  1. Medical Research Council, Edinburgh, Scotland

    John R. Gosden

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© 1994 Humana Press Inc, Totowa, NJ

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Porteous, D.J. (1994). Protocols for Chromosome-Mediated Gene Transfer. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:353

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  • DOI: https://doi.org/10.1385/0-89603-289-2:353

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-289-7

  • Online ISBN: 978-1-59259-516-7

  • eBook Packages: Springer Protocols

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