Oligonucleotide Primed in Situ DNA Synthesis (PRINS)

An Alternative to in Situ Hybridization for Gene Mapping and the Investigation of Genome Organization
  • John R. Gosden
Part of the Methods in Molecular Biology™ book series (MIMB, volume 29)

Abstract

The technique of in situ hybridization has come of age, it being more than 21 years since the first descriptions of the procedure were published (1). In that time, the capabihties of the method have expanded enormously. In the early years, the sensitivity (minimum size of target capable of being detected) was restricted to highly repeated DNA sequences, such as satellite DNAs, and detection was exclusively by autoradiography of radioisotopic label (e.g., ref. 2). In time, it became possible to map unique sequences (e.g., ref. 3), but this was a tedious procedure involving the analysis of grain distribution over large numbers of metaphase spreads, in order to distinguish specific hybridization signal from background grains.

Keywords

Satellite DNAs Skim Milk Powder Trisodium Citrate Metaphase Spread Optimum Annealing Temperature 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc, Totowa, NJ 1994

Authors and Affiliations

  • John R. Gosden
    • 1
  1. 1.Medical Research Council, Human Genetics UnitWestern General HospitalEdinburghScotland

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