Abstract
Immunofluorescence has proven to be a powerful technique for the in situ localization of antigens at a cytological level. Whether utilized to determine the cellular distribution of known antigens using defined antibodies, to aid in the characterization of novel antigens recognized, for example, by autoimmune sera, or to screen monoclonal antibodies for their cognate antigens, the immunofluorescence method has the merit of combining high sensitivity with the ability apparently to “visualize” antigens directly.
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© 1994 Humana Press Inc, Totowa, NJ
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Jeppesen, P. (1994). Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:253
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DOI: https://doi.org/10.1385/0-89603-289-2:253
Publisher Name: Humana Press
Print ISBN: 978-0-89603-289-7
Online ISBN: 978-1-59259-516-7
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