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Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity

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Chromosome Analysis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 29))

Abstract

The introduction of chromosome banding techniques about 20 years ago has not only provided a very powerful tool for chromosome identification, but has also highlighted structural and functional differences between different chromosomal segments (1). Restriction endonucleases (RE) have been widely used to study some aspects of chromosome organization (2–4). The original methods, involving digestion followed by Giemsa or ethidium bromide (EB) staining, lack both specificity and sensitivity. It is now clear that the Giemsa/EB staining does not necessarily represent the distribution of restriction sites along the chromosomes; other factors, such as the accessibility of the REs to the targets or the extractability of the DNA fragments produced, need to be considered.

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Author information

Authors and Affiliations

  1. Unidad de Genetica, Universidad Autoademia de Madrid, Spain

    Joaquina de la Torre

  2. Medical Research Council, Human Genetics Unit, Western General Hospital, Edinburgh, Scotland

    Adrian T. Sumner

Authors
  1. Joaquina de la Torre
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  2. Adrian T. Sumner
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Editor information

Editors and Affiliations

  1. Medical Research Council, Edinburgh, Scotland

    John R. Gosden

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© 1994 Humana Press Inc, Totowa, NJ

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Torre, J.d.l., Sumner, A.T. (1994). Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:123

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  • DOI: https://doi.org/10.1385/0-89603-289-2:123

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-289-7

  • Online ISBN: 978-1-59259-516-7

  • eBook Packages: Springer Protocols

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