Immunocytochemical Techniques Applied to Meiotic Chromosomes

  • Christa Heyting
  • Axel J. J. Dietrich
  • J. de Hans Jong
  • Edgar Hartsuiker
Part of the Methods in Molecular Biology™ book series (MIMB, volume 29)

Abstract

During the prophase of the first meiotic division, homologous chromosomes align, pair, recombine, and segregate. These processes are accompanied by the appearance of meiosis-specific structures. Chromosome alignment is accompanied by the formation of axial elements along each chromosome (1); in meiocytes of some species, fibers appear to pull the axial elements of homologous chromosomes together (2). Alignment is followed by a process called synapsis: The axes of homologous chromosomes are closely apposed, and a tripartite structure, called the synaptonemal complex (SC), is formed. It consists of the axial elements of the homologs, connected by transversal filaments, and a central element that is formed on the transversal filaments between the axes (1).

Keywords

H2O2 Sucrose Glycerol Dust Acetone 

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Copyright information

© Humana Press Inc, Totowa, NJ 1994

Authors and Affiliations

  • Christa Heyting
    • 1
  • Axel J. J. Dietrich
    • 1
  • J. de Hans Jong
    • 2
  • Edgar Hartsuiker
    • 2
  1. 1.Institute of Human GeneticsUniversity of Amsterdam, Academic Medical CentreAmsterdamThe Netherlands
  2. 2.Department of GeneticsAgricultural UniversityWageningenThe Netherlands

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