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Purification of the human TATA-Binding Protein, TBP

  • Protocol
In Vitro Transcription and Translation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 37))

Abstract

The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1, 2). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP facilitates the assembly of RNA polymerase II and other initiation factors (TFIIA,-B,-E,-F,-G/J, and H) into an active initiation complex (3).

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Authors and Affiliations

  1. Center for Gene Regulation, Department of Molecular and Cell Biology, The Pennsylvania State University, University Park, PA

    B. Franklin Pugh

Authors
  1. B. Franklin Pugh
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Editor information

Editors and Affiliations

  1. Monash University Clayton, Victoria, Australia

    Martin J. Tymms

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© 1995 Humana Press Inc.

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Pugh, B.F. (1995). Purification of the human TATA-Binding Protein, TBP. In: Tymms, M.J. (eds) In Vitro Transcription and Translation Protocols. Methods in Molecular Biology, vol 37. Humana Press. https://doi.org/10.1385/0-89603-288-4:359

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  • DOI: https://doi.org/10.1385/0-89603-288-4:359

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-288-0

  • Online ISBN: 978-1-59259-524-2

  • eBook Packages: Springer Protocols

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