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Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro

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In Vitro Transcription and Translation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 37))

Abstract

Much of our current understanding of the mechanisms of cellular protein targeting and membrane translocation of proteins derives from studies on mitochondria. The mitochondrial import process has been studied both in whole cells (including extensive use of mutants, particularly in yeast) and in isolated mitochondria. This chapter will discuss the technical details of in vitro import studies using isolated yeast mitochondria.

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Authors and Affiliations

  1. Centre for Molecular Biology and Medicine and Department of Biochemistry, Monash University, Clayton, Victoria, Australia

    Ruby H. P. Law & Phillip Nagley

Authors
  1. Ruby H. P. Law
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  2. Phillip Nagley
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Editor information

Editors and Affiliations

  1. Monash University Clayton, Victoria, Australia

    Martin J. Tymms

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© 1995 Humana Press Inc.

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Law, R.H.P., Nagley, P. (1995). Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro. In: Tymms, M.J. (eds) In Vitro Transcription and Translation Protocols. Methods in Molecular Biology, vol 37. Humana Press. https://doi.org/10.1385/0-89603-288-4:293

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  • DOI: https://doi.org/10.1385/0-89603-288-4:293

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-288-0

  • Online ISBN: 978-1-59259-524-2

  • eBook Packages: Springer Protocols

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