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In Vitro Transcription and Translation Protocols pp 199–213Cite as

The Xenopus Egg Extract Translation System

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 37))

Abstract

A full analysis of the posttranslational modifications that a given protein undergoes during transit through the secretory pathway may, in some cases, only be performed by analysis of the natural protein, expressed in its normal tissue.

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References

  1. Matthews, G. M. and Colman, A. (1991) A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs. Nucleic Acids Res. 19, 6405–6412.

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  2. Murray, A. W. (1991) Cell cycle extracts, in Methods in Cell Biology, vol. 36 (Kay, B. K. and Peng, H. eds.), Academic, San Diego, pp. 581–605.

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  3. Kreig, P. A. and Melton, D. A. (1984) Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Rex 12, 7057–7070.

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  4. Colman, A. (1984) Translation of eukaryotic messenger RNA in Xenopus oocytes, in Transcription and Translation-A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, pp. 271–302.

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Author information

Authors and Affiliations

  1. School of Biochemistry, University of Birmingham, UK

    Glenn M. Matthews

Authors
  1. Glenn M. Matthews
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  2. Alan Colman
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Editor information

Editors and Affiliations

  1. Monash University Clayton, Victoria, Australia

    Martin J. Tymms

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© 1995 Humana Press Inc.

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Cite this protocol

Matthews, G.M., Colman, A. (1995). The Xenopus Egg Extract Translation System. In: Tymms, M.J. (eds) In Vitro Transcription and Translation Protocols. Methods in Molecular Biology, vol 37. Humana Press. https://doi.org/10.1385/0-89603-288-4:199

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  • DOI: https://doi.org/10.1385/0-89603-288-4:199

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-288-0

  • Online ISBN: 978-1-59259-524-2

  • eBook Packages: Springer Protocols

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