Abstract
Subtraction-hybridization cDNA libraries (1–4) are libraries enriched for sequences representing mRNAs whose expression in one biological source (e.g., tissues, cell lines) is different than in a second source. Single-stranded cDNAs from both sources are allowed to hybridize so that sequences common to the two sources will anneal. The annealed, double-stranded DNAs are “subtracted” from the hybridization solution, leaving a population of cDNA molecules enriched for sequences preferentially expressed (or repressed) in the biological source of interest. Figure 1 diagrammatically represents the scheme for subtraction hybridization currently employed in our laboratory.
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References
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© 1995 Humana Press Inc.
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Schweinfest, C.W., Nelson, P.S., Graber, M.W., Demopoulos, R.I., Papas, T.S. (1995). Subtraction Hybridization cDNA Libraries. In: Tymms, M.J. (eds) In Vitro Transcription and Translation Protocols. Methods in Molecular Biology, vol 37. Humana Press. https://doi.org/10.1385/0-89603-288-4:13
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DOI: https://doi.org/10.1385/0-89603-288-4:13
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