Abstract
The chick embryo has been used for the study of experimental teratogenicity by various techniques (1–4). This chapter describes a method developed by Jelinek (5,6) that combines a standardized technique (CHEST I) with other techniques (CHEST II); it allows the administration of small amounts of test compound and the measurement of a quantitative endpoint (CHEST I).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
McLaughlin, J., Jr., Marliac, J., Verret, M. J., Mutcler, M K, and Fitzhugh, O. G (1963) The injection of chemicals into the yolk sac of fertile eggs prior to incubation as a toxicity test. Toxicol. Appl. Pharm. 5, 760–771.
Gebhardt, D. O. E. (1972) The use of the chick embryo in applied teratology, in Advances in Teratology, vol. 5 (Woollam, D. H M., ed), Academy, New York, pp. 97–111
Verrett, M. J., Scott, W. F., Reynaldo, E. F., Alterman, E. K., and Thomas, C A. (1980) Toxicity and teratogenicity of food additive chemicals in the developing chicken embryo. Toxicol. Appl. Pharm. 56, 265–273.
Fisher, M and Schoenwolf, G. C. (1983) The use of early chick embryos in experimental embryology and teratology: improvements in standard procedures. Teratology 27, 65–72.
Jelinek, R. (1977) The chick embryotoxicity screening test (CHEST), in Methods in Prenatal Toxicology, (Neubert, D, Merker, H. J., and Kwasigroch, T. E., eds.), G Thieme, Stuttgart, pp. 381–386.
Jelinek, R, Peterka, M, and Rychter, Z (1985) Chick embryotoxicity screening test—130 substances tested Ind J Exp. Biol. 23, 588–595
Hamburger, V and Hamilton, H. L. (1951) A series of normal stages in the development of the chick embryo. J. Morphol. 88, 49–92.
Hamburger, V. (1960) A Manual of Experimental Embryology, rev ed, University of Chicago Press, Chicago.
Vesely, D, Vesela, D., and Jelinek, R (1982) Nineteen mycotoxins tested on chicken embryos. Toxicol Lett 13, 239–245.
Vesely, D., Vesela, D, and Jelinek, R (1984) Use of chick embryo in screening for toxin-producing fungi Mycopathologia 88, 135–140.
Jelinek, R and Rychter, Z. (1979) Morphogenetic systems and the central phenomena of teratology, in Advances in the Study of Birth Defects, vol. 11, Teratological Testing (Persaud, T V. M., ed.), University Park Press, Baltimore, MD, pp. 41–67
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Humana Press Inc , Totowa, NJ
About this protocol
Cite this protocol
Davies, W.J., Freeman, S.J. (1995). Chick Embryotoxicity Screening Test (CHEST I and II). In: O’Hare, S., Atterwill, C.K. (eds) In Vitro Toxicity Testing Protocols. Methods in Molecular Biology™, vol 43. Humana Press. https://doi.org/10.1385/0-89603-282-5:307
Download citation
DOI: https://doi.org/10.1385/0-89603-282-5:307
Publisher Name: Humana Press
Print ISBN: 978-0-89603-282-8
Online ISBN: 978-1-59259-530-3
eBook Packages: Springer Protocols