Abstract
In the search for valid alternatives to animals in assessing the acute toxicity of substances foreign to humans, the use of cells in vitro is a sound proposition. The validity of any in vitro model is limited, however, because the physiological, immunological, and inflammatory conditions in vivo cannot be reproduced in vitro (1). In fact, these elements also mean that difficulties occur when extrapolating results from whole animals to events in humans.
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References
Kemp, R Meredith, R W. J., Gamble, S., and Frost, M (1983) A rapid cell culture technique for assessing the toxicity of detergent-based products in vitro as a possible screen for eye irritancy in vivo Cytobios 36, 153–159.
Dixon, K. C. (1967) Events in dying cells. Proc. Roy Soc. Med 60, 271–275
Malinin, T I and Perry, P. (1967) A review of tissue and organ viability assays. Cryobiology 4, 104–115.
Trump, F., Valigorsky, J., and Dees, J. (1973) The modernisation of the autopsy applications of ultrastructural and biochemical methods to human disease. Med. Coll VA Q. 9, 323–333
Cornelis, M., Dupont, C., and Wepierre, J. (1991) In vitro cytotoxicity tests on cultured human skin fibroblasts to predict the irritation potential of surfactants ATLA 19, 324–336.
Kemp, R., Jones, B. M., Cunningham, I., and James, M M (1967) Quantitative investigation on the effect of puromycin on the aggregation of trypsin-and versene-dissociated chick fibroblast cells J. Cell Sci 2, 323–340.
Reinhardt, A, Pelli, D. A., and Zbinden, G. (1985) Interpretation of cell toxicity data for the estimation of potential irritation. Fed Chem. Toxicol. 23, 247–252.
Parish, W. E. (1985) Relevance of in vitro tests to in vivo acute skin inflammation-potential in vitro applications of skin keratome slices, neutrophils, fibroblasts, mast cells and macrophages. Fed Chem. Toxicol 23, 275–288.
Baserga, R (1989) Cell Growth and Division, IRL, Oxford, pp. 39–41.
Kemp, R., Cross, D. M, and Meredith, R. W J (1988) Comparison of cell death and adenosine triphosphate as indicators of acute toxicity in vitro Xenobiotica 18, 633–639.
Rotman, B. and Papermaster, W. (1966) Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters Proc. Natl Acad. Sci. USA 55, 134–141.
Gabby, E. J. and Wilson, W D. (1978) Intercalating agents as probes of chromatin structures Meth Cell. Biol. 18, 351–384.
Burns, V. W. (1972) Localization and molecular characteristics of fluorescent complexes of ethidium bromide in the cell Exp. Cell Res. 75, 200–206
Edidin, M. (1970) A rapid quantitative fluorescence assay for cell damage by cytotoxic antibodies J Immunol. 104, 1303–1306
Takasugi, M. (1971) An improved fluorochromatic cytotoxic test Transplantation 12, 148–151.
Balls, M. and Horner, S. A. (1985) The FRAME interlaboratory programme on in vitro cytotoxicity. Fed. Chem. Toxicol. 23, 209–213
Freshney, R. I. (1994) Culture of Animal Cells and Manual of Basic Techniques, 3rd ed., Liss, New York, 492 p.
Kemp, R. Meredith, R. W. J., and Gamble, S. H. (1985) Toxicity of commercial products on cells in suspension culture, a possible screen for the Draize Eye Irritation Test. Fed. Chem. Toxicol. 23, 267–270.
Ulrich, K. and Moore, G. E (1965) A vibrating mixer for agitation of suspension cultures of mammalian cells. Biotech. Bioeng. 7, 417.
Krewski, D. and Franklin, S., eds. (1991) Statistics in Toxicology, Gordon and Breach, New York.
Bliss, I. (1937) The calculation of the time-mortality curve. Ann. Appl. Biol. 24, 815–852.
Litchfield, J. T., Jr. (1949) A method of time-per cent effect curves J. Pharm. Exp. Ther. 97, 399–406
Litchfield, J. T., Jr. and Wilcoxon, F (1949) A simplified method of evaluating dose-effect curves. J. Pharm Exp. Ther. 96, 99–115
Kemp, R. B. Cross, D. M, and Meredith, R W. J. (1986) Adenosine triphosphate as an indicator of cellular toxicity in vitro. Fed. Chem. Toxicol. 24, 465–466.
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© 1995 Humana Press Inc , Totowa, NJ
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Kemp, R.B. (1995). Cytotoxicity in an Anchorage-Independent Fibroblast Cell Line Measured by a Combination of Fluorescent Dyes. In: O’Hare, S., Atterwill, C.K. (eds) In Vitro Toxicity Testing Protocols. Methods in Molecular Biology™, vol 43. Humana Press. https://doi.org/10.1385/0-89603-282-5:211
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DOI: https://doi.org/10.1385/0-89603-282-5:211
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