Abstract
In this test, kidney-derived cells are cultured in the presence of test compounds whose cytotoxicity is then determined by the Neutral Red method, and serves as an indicator of potential nephrotoxicity (1). Healthy LLC-RK1 cells (an established cell line, ATCC CCL) maintained in culture continuously divide and multiply over time but still retain certain characteristics of kidney cells in vivo (2). Compounds that have a deleterious effect on these cells may, therefore, be considered as potential in vivo nephrotoxins. In this test system, harmful effects on cell viability are determined by monitoring the uptake of the vital dye Neutral Red into the lysosomes of healthy cells.
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References
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© 1995 Humana Press Inc , Totowa, NJ
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White, D.J., Seaman, C. (1995). LLC-RK1 Cell Screening Test for Nephrotoxicity. In: O’Hare, S., Atterwill, C.K. (eds) In Vitro Toxicity Testing Protocols. Methods in Molecular Biology™, vol 43. Humana Press. https://doi.org/10.1385/0-89603-282-5:11
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DOI: https://doi.org/10.1385/0-89603-282-5:11
Publisher Name: Humana Press
Print ISBN: 978-0-89603-282-8
Online ISBN: 978-1-59259-530-3
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