Abstract
The cytotoxic effect of chemicals on cells in culture is measured by the change in total cell protein (Kenacid Blue R dye binding method). Healthy 3T3-L1 cells (an established cell-line, ATCC CCL92.1), when maintained in culture continuously divide and multiply over time. The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with this process and, thus, result in a reduction of the growth rate as reflected by cell number. The degree of inhibition of growth, related to the concentration of the test compound, provides an indication of toxicity.
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© 1995 Humana Press Inc , Totowa, NJ
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Clothier, R.H. (1995). The FRAME Cytotoxicity Test (Kenacid Blue). In: O’Hare, S., Atterwill, C.K. (eds) In Vitro Toxicity Testing Protocols. Methods in Molecular Biology™, vol 43. Humana Press. https://doi.org/10.1385/0-89603-282-5:109
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DOI: https://doi.org/10.1385/0-89603-282-5:109
Publisher Name: Humana Press
Print ISBN: 978-0-89603-282-8
Online ISBN: 978-1-59259-530-3
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