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Oligonucleotide Phosphorofluoridates and Fluoridites

  • Protocol
Protocols for Oligonucleotides and Analogs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 20))

Abstract

Fluoro derivatives of phosphorus are of great importance in the chemistry and biochemistry of both elements. The incorporation of fluorine into biomolecules has frequently resulted in a remarkable change of biological properties. Simple phosphofluoridates and their structural analogs are the classical inhibitors of serine proteases (1a). It can be envisaged that a combination of nucleoside fragments with the phosphorofluoridate moiety could result in new properties with respect to selectivity of interaction with the active site of the serine hydroxy group. On the other hand, nucleotides containing a P—F linkage when incorporated into oligonucleotides could be used in the studies of biological functions of nucleic acids, including possibilities connected with the chirality of the phosphorofluoridate moiety. New phosphorofluoridate analogs of nucleotides could also be useful in studying the metabolic processes (1b) by noninvasive NMR imaging techniques taking advantage of 100% abundance of 19F nucleus and its high inherent NMR sensitivity.

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Author information

Authors and Affiliations

  1. Max-Planck-Institut für Experimentelk Medizin, Gottingen, Germany

    Wojciech Dabkowski, Jan Michahki & Friedrich Cramer

Authors
  1. Wojciech Dabkowski
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  2. Jan Michahki
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  3. Friedrich Cramer
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Editor information

Editors and Affiliations

  1. Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts

    Sudhir Agrawal

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© 1993 Humana Press Inc., Totowa, NJ

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Dabkowski, W., Michahki, J., Cramer, F. (1993). Oligonucleotide Phosphorofluoridates and Fluoridites. In: Agrawal, S. (eds) Protocols for Oligonucleotides and Analogs. Methods in Molecular Biology, vol 20. Humana Press. https://doi.org/10.1385/0-89603-281-7:245

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  • DOI: https://doi.org/10.1385/0-89603-281-7:245

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-281-1

  • Online ISBN: 978-1-59259-507-5

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