Abstract
An innovative variation of fluorescence in situ hybridization (FISH) allows preparation of single-stranded chromatids for determination of chromosomal orientation and strand direction of DNA sequences (1). Standard fluorescent in situ hybridization methods rely on nucleotide base pairing between a labeled probe and the complementary chromosomal target sequence. Both probe and target DNAs must be functionally single-stranded at the start of the procedure. Single-stranded probes can be constructed or prepared, but the chromosomal DNA must be made single-stranded by denaturation. Because both strands are present in both chromatids of each chromosome, even single-stranded probes will hybridize to both chromatids of the target chromosome.
Reference
Goodwin, E H and Meyne, J (1993) Strand-specific FISH reveals orientation of chromosome 18 alphoid DNA Cytogenet Cell Genet 63, 126,127
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© 1994 Humana Press Inc.
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Meyne, J., Goodwin, E.H. (1994). Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA Sequences. In: Choo, K.H.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 33. Humana Press. https://doi.org/10.1385/0-89603-280-9:141
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DOI: https://doi.org/10.1385/0-89603-280-9:141
Publisher Name: Humana Press
Print ISBN: 978-0-89603-280-4
Online ISBN: 978-1-59259-520-4
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