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Free Chromatin Mapping by FISH

  • Protocol
In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 33))

Abstract

Different strategies have been introduced to facilitate characterization of complex genomes; the techniques include molecular cloning, agarose gel electrophoresis and blot-hybridization analysis, DNA sequencing, construction of somatic cell hybrid lines, in situ hybridization, as well as genetic (linkage) analysis. Because of their wide application, numerous improvements have been made in each of these areas in recent years, for example, the use of pulsed-field gel electrophoresis (PFGE), yeast artificial chromosome (YAC), and fluorescence in situ hybridization (FISH) (1–4). Obviously, no single method can provide all the information required for detailed analysis of genomes with greater than several billion base pairs (bp) and each method has its limitation in range of resolution. A combination of different techniques is therefore necessary for any large-scale gene mapping endeavors.

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Author information

Authors and Affiliations

  1. Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada

    Henry H. Q. Heng & Lap-Chee Tsui

  2. Department of Genetics, The Hospital for Sick Children, Toronto, Ontario, Canada

    Henry H. Q. Heng & Lap-Chee Tsui

Authors
  1. Henry H. Q. Heng
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  2. Lap-Chee Tsui
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Editor information

Editors and Affiliations

  1. Murdoch Institute, Melbourne, Australia

    K. H. Andy Choo

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© 1994 Humana Press Inc.

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Heng, H.H.Q., Tsui, LC. (1994). Free Chromatin Mapping by FISH. In: Choo, K.H.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 33. Humana Press. https://doi.org/10.1385/0-89603-280-9:109

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  • DOI: https://doi.org/10.1385/0-89603-280-9:109

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-280-4

  • Online ISBN: 978-1-59259-520-4

  • eBook Packages: Springer Protocols

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