Detection of Virus Nucleic Acids by Radioactive and Nonisotopic In Situ Hybridization
Unlike other applications of in situ hybridization (ISH),where the target is likely to be chromosomal DNA or RNA transcripts, the target in studies to detect viral nucleic acids may represent double-or single-stranded (ds or ss) DNA or RNA (representative of viral genomes), nucleic acid replicative intermediates or mRNA. Consequently, the sample may require an optional denaturation step to distinguish ds and ss nucleic acids, and/or a prior nuclease digestion step.
KeywordsFormalin Hepatitis Dust MgCl2 Paraffin
- 1.Gowans, E J., Jilbert, A R, and Burrell, C J (1989) Detection of specific DNA and RNA sequences In tissues and cells by in situ hybridization, in Nucleic Acid Probes (Symons, R H., ed), CRC, Boca Raton, FL, pp, 130–158.Google Scholar
- 7.Simmons, A. and Nash, A A. (1984) Zosteriform spread of herpes simplex virus as a model of recrudescence and its use to investigate the role of immune cells in prevention of recurrent disease J Virol 53, 944–948.Google Scholar
- 10.Britton, R J. and Davidson, E. H (1986) Hybridization strategy, in Nucleic Acid Hybridization. A Practical Approach (Rickwood, D and Homes, B D, eds), IRL, Oxford, UK, pp. 3–15Google Scholar
- 13.Davis, L G., Dibner, M D, and Battey, J F. (1986) Hybridization with synthetic end-labelled probes, in Basic Methods in Molecular Biology, Elsevier, New York, pp 75–78.Google Scholar
- 14.Rogers, A. W (1979) Techniques for Autoradiography, 3rd ed, Elsevier, North Holland.Google Scholar