Detection of Virus Nucleic Acids by Radioactive and Nonisotopic In Situ Hybridization

  • Eric J. Gowans
  • Keril Blight
  • Jane Arthur
  • Geoffrey D. Higgins
Part of the Methods in Molecular Biology™ book series (MIMB, volume 33)

Abstract

Unlike other applications of in situ hybridization (ISH),where the target is likely to be chromosomal DNA or RNA transcripts, the target in studies to detect viral nucleic acids may represent double-or single-stranded (ds or ss) DNA or RNA (representative of viral genomes), nucleic acid replicative intermediates or mRNA. Consequently, the sample may require an optional denaturation step to distinguish ds and ss nucleic acids, and/or a prior nuclease digestion step.

Keywords

Formalin Hepatitis Dust MgCl2 Paraffin 

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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Eric J. Gowans
    • 1
  • Keril Blight
    • 2
  • Jane Arthur
    • 1
  • Geoffrey D. Higgins
    • 1
  1. 1.Division of Medical VirologyInstitute of Medical and Veterinary ScienceAdelaideAustralia
  2. 2.Department of Microbiology and ImmunologyUniversity of AdelaideAdelaideAustralia

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