ELISA pp 63-97 | Cite as

Stages in ELISA

  • John R. Crowther
Part of the Methods in Molecular Biology™ book series (MIMB, volume 42)


This chapter gives general information on common practical features of the ELISA, featuring the main elements of:
  1. 1.

    The adsorption of antigen or antibody to the plastic solid-phase.

  2. 2.

    The addition of the test sample and subsequent reagents.

  3. 3.

    The incubation of reactants.

  4. 4.

    The separation of bound and free reactants by washing.

  5. 5.

    The addition of enzyme-labeled reagent.

  6. 6.

    The addition of enzyme detection system (color development).

  7. 7.

    The visual or spectrophotometric reading of the assay.



Colored Product Spectrophotometric Reading Bromocresol Purple Initial Dilution Plate Data 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • John R. Crowther
    • 1
  1. 1.Institute for Animal HealthWokingUK

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