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Chaperonins in Phage Display of Antibody Fragments

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Antibody Engineering Protocols

Part of the book series: Methods In Molecular Medicine™ ((MIMB,volume 51))

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Abstract

The display of antibody fragments on the surface of filamentous bacteriophages (1–7) constitutes a powerful system for the selection of molecules with desired specificities. In phage display, the antibody fragment is coupled to the minor coat protein (protein3) of bacteriophage M13 phage and is, in this way, both anchored in the phage capsid and exposed on the phage surface, linking specificity and genetic information. This permits direct isolation and sequence determination of the gene encoding the antibody fragment. The gene can subsequently be exposed to further engineering and selection in order to improve affinity and specificity.

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Author information

Authors and Affiliations

  1. Department of Immunotechnology, Lund University, Lund, Sweden

    Eskil Söderlind, Marta Dueñas & Carl A. K. Borrebaeck

Authors
  1. Eskil Söderlind
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  2. Marta Dueñas
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  3. Carl A. K. Borrebaeck
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Editor information

Editors and Affiliations

  1. Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE

    Sudhir Paul

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© 1995 Humana Press Inc.

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Söderlind, E., Dueñas, M., Borrebaeck, C.A.K. (1995). Chaperonins in Phage Display of Antibody Fragments. In: Paul, S. (eds) Antibody Engineering Protocols. Methods In Molecular Medicine™, vol 51. Humana Press. https://doi.org/10.1385/0-89603-275-2:343

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  • DOI: https://doi.org/10.1385/0-89603-275-2:343

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-275-0

  • Online ISBN: 978-1-59259-538-9

  • eBook Packages: Springer Protocols

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