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Cloning and Bacterial Expression of an Esterolytic sFv

  • Protocol
Antibody Engineering Protocols

Part of the book series: Methods In Molecular Medicine™ ((MIMB,volume 51))

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Abstract

In 1988 the first report on the expression of a fully functional recombinant Fab in E. coli (1) was one of the initiating events in the development of the field of antibody engineering. Shortly after came the report of the cloning and expression of antibody-variable domains as single-chain Fv or sFv (2). This provided a method for rapid cloning of stable antibody domains from hybridoma cells into E. coli in a fully functional form. More recent developments in the field, such as expression and selection of libraries of sFv or Fab on phage particles, have allowed the de novo generation of antibodies with binding properties equivalent to or, in some cases, better than those derived by traditional hybridoma methods (3).

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Author information

Authors and Affiliations

  1. IGEN Inc., Rockville, MD

    Rodger G. Smith, Mark T. Martin & Rosa Sanchez

  2. Proneuron, Inc., Rockville, MD

    John H. Kenten

Authors
  1. Rodger G. Smith
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  2. Mark T. Martin
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  3. Rosa Sanchez
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  4. John H. Kenten
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Editor information

Editors and Affiliations

  1. Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE

    Sudhir Paul

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© 1995 Humana Press Inc.

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Smith, R.G., Martin, M.T., Sanchez, R., Kenten, J.H. (1995). Cloning and Bacterial Expression of an Esterolytic sFv . In: Paul, S. (eds) Antibody Engineering Protocols. Methods In Molecular Medicine™, vol 51. Humana Press. https://doi.org/10.1385/0-89603-275-2:297

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  • DOI: https://doi.org/10.1385/0-89603-275-2:297

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-275-0

  • Online ISBN: 978-1-59259-538-9

  • eBook Packages: Springer Protocols

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