Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation

  • Su-jun Deng
  • C. Roger MacKenzie
  • Saran A. Narang
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 51)

Abstract

The application of phage display to the screening of antibody V-gene libraries promises to replace animal immunization and hybridoma technology as a means of antibody generation (1,2). Synthetic libraries offer the potential for expanding the immune system repertoire and designing libraries for particular sets of antigens. We have developed a method for the generation of synthetic antibody gene libraries by which spiked oligonucleotides are assembled by a ligase chain reaction protocol (3). This is a synthetic adaptation of a reaction that was previously used as a diagnostic tool (4,5).

Keywords

Epoxy Electrophoresis MgCl2 Ampicillin Tetracycline 

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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Su-jun Deng
    • 1
  • C. Roger MacKenzie
    • 1
  • Saran A. Narang
    • 1
  1. 1.Institute for Biological SciencesNational Research Council of CanadaOttawaCanada

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