Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation

  • Su-jun Deng
  • C. Roger MacKenzie
  • Saran A. Narang
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 51)


The application of phage display to the screening of antibody V-gene libraries promises to replace animal immunization and hybridoma technology as a means of antibody generation (1,2). Synthetic libraries offer the potential for expanding the immune system repertoire and designing libraries for particular sets of antigens. We have developed a method for the generation of synthetic antibody gene libraries by which spiked oligonucleotides are assembled by a ligase chain reaction protocol (3). This is a synthetic adaptation of a reaction that was previously used as a diagnostic tool (4,5).


Phage Display Affinity Maturation Affinity Measurement scFv Gene Ligase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Su-jun Deng
    • 1
  • C. Roger MacKenzie
    • 1
  • Saran A. Narang
    • 1
  1. 1.Institute for Biological SciencesNational Research Council of CanadaOttawaCanada

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