Catalytic Antibodies

Structure and Possible Applications
  • Howard Amital
  • Ilan Tur-Kaspa
  • Zeev Tashma
  • Israel Hendler
  • Yehuda Shoenfeld
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 51)


Despite major progress in various fields of chemical and molecular biology, generation of a synthetic molecule that possesses the catalytic qualities of enzymes has not been achieved. In order to overcome this obstacle, numerous attempts have been carried out to synthesize antibodies that express catalytic activity.


Systemic Lupus Erythematosus Boronic Acid Organophosphorus Compound Chemical Warfare Agent Catalytic Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Pollack, S. J., Jacobs, W. J., and Schultz, P.G. (1986) Selective chemical catalysis by an antibody. Science 234, 1570–1573.PubMedCrossRefGoogle Scholar
  2. 2.
    Tramontano, A., Janda, K. D., and Lerner, R. A. (1986) Chemical reactivity of an antibody binding site elicited by mechanistic design of a synthetic antigen. Proc. Natl. Acad. Sci. USA 83, 6736–6740.PubMedCrossRefGoogle Scholar
  3. 3.
    Lerner, R. A., Benkovic, S. J., and Schultz, P. G. (1991) At the crossroad of chemistry and immunology catalytic antibodies. Science 252, 658–667.CrossRefGoogle Scholar
  4. 4.
    Paul, S., Sun, M., Mody, R., Tewary, H. K., Stemmer, P., Massey, R J., Gianferrara, T., Mehrotra, S., Dreyer, T., Meldal, M., and Tramontano, A. (1992) Peptidolytic monoclonal antibody elicited by a neuropeptide. J. Biol. Chem. 267, 13,142–13,145PubMedGoogle Scholar
  5. 5.
    Tawfik, D. S., Eshhar, Z., and Green, B. S. (1994) Catalytic antibodies: a critical assessment. Mol. Biotechnol. 1, 87–103.PubMedCrossRefGoogle Scholar
  6. 6.
    Pollack, S. J., Nakayama, G. R., and Schultz, P. G. (1988) Introduction of nucleophiles and spectrophotometric probes into antibody combining sites. Science 242, 1038–1040.PubMedCrossRefGoogle Scholar
  7. 7.
    Pollack, S. J., Nakayama, G. R., and Schultz, P. G. (1989) Design of catalytic antibodies. Methods Enzymol. 178, 551–568.PubMedCrossRefGoogle Scholar
  8. 8.
    Iverson, B. L. and Lerner, R. A. (1989) Sequence specific peptide cleavage catalyzed by an antibody. Science 243, 1184–1188.PubMedCrossRefGoogle Scholar
  9. 9.
    Lerner, R. A. and Tramontano, A. (1988) Catalytic antibodies. Sci. Am. 258, 58–60.PubMedCrossRefGoogle Scholar
  10. 10.
    Dunn, M. A. and Sidell, F. R. (1989) Progress in medical defence against nerve agents. J. Am. Med. Assoc. 262, 649–663.CrossRefGoogle Scholar
  11. 11.
    Koelle, G. B. (1981) Organophosphate poisoning: an overview. Fund. Appl. Toxic 1, 129–134.CrossRefGoogle Scholar
  12. 12.
    Lenz, D. E., Brimfield, A. A., Hunter, K. W., Benschop, H. P., de Jong, L. P., Van Dijk, C., and Cloro, T. R. (1984) Studies using monoclonal antibody against soman. Fund. Appl. Toxicol. 45, 5156–5164.Google Scholar
  13. 13.
    Brimfield, A. A., Hunter, K. W., Lenz, D. E., Benschop, H. P., Van Dijk, C., and de Jong, L. P. (1985) Structural and stereochemical specificity of mouse monoclonal antibodies to the organophosphorus cholinesterase inhibitor soman. Mol. Pharmacol. 28, 32–39.PubMedGoogle Scholar
  14. 14.
    Glikson, M., Arad-Yellin, R., Ghozi, M., Raveh L., Green, B. S., and Eshhar, Z. (1992) Characterization of soman-binding antibodies raised against soman analogs. Mol. Immunol. 29, 903–910.PubMedCrossRefGoogle Scholar
  15. 15.
    Lenz, D. E., Yourick, J. J., Dawson, J. S., and Scott, J. (1992) Monoclonal antibodies against soman. characterization of soman stereoisomers. Immunol. Lett. 31, 131–136.PubMedCrossRefGoogle Scholar
  16. 16.
    Buenafe, A. C. and Rittenberg, M. B. (1987) Combining site specific of monoclonal antibodies to the organophosphate hapten soman. Mol. Immunol. 24, 401–407.PubMedCrossRefGoogle Scholar
  17. 17.
    Buenafe, A. C., Makowski, F. F., and Rittenberg, M. B. (1989) Molecular analysis and fine specificity of antibodies against an organophosphorus hapten. J. Immunol. 143, 539–545.PubMedGoogle Scholar
  18. 18.
    Dunn, M. A. and Sidell, F. R. (1989) Progress in medical defence against nerve agents. J. Am. Med. Assoc. 262, 649–653.CrossRefGoogle Scholar
  19. 19.
    Sternberg, L. A., Sim, V. M., and Kavanagh, W. G. (1972) A vaccine against organophosphorus poisoning. Army Conference Proc. 3, 429.Google Scholar
  20. 20.
    Rong, K. T. and Zhang, L. J. (1990) Immunologic protection against VS intoxication in experimental animals. Pharmacol. Toxicol. 67, 255–259.PubMedCrossRefGoogle Scholar
  21. 21.
    Brimfield, A. A. and Lenz, D. E. (1991) Progress in the production of catalytic antibodies as biological scavengers for organophosphorus poisons, in Proceedings of the 1991 Medical Defence Bioscience Review, US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, pp. 431–438.Google Scholar
  22. 22.
    Brimfield, A. A., Lenz, D. E., Maxwell, D. M., and Broomfield, C. A. (1993) Catalytic antibodies hydrolyzing organophosphorus esters. Chem.-Biol. Interactions 87, 95–102.CrossRefGoogle Scholar
  23. 23.
    Gentry, M. K., Saxena, A., Asham, Y., and Doctor, B. P. (1993) Immunochemical characterization of anti-acetylcholinesterase inhibitory monoclonal antibodies. Chem. Biol. Interactions 87, 227–231.CrossRefGoogle Scholar
  24. 24.
    Izadyar, L., Friboulet, A., Remy, M. H., Roseto, A., and Thomas, D. (1993) Monoclonal anti-idiotypic antibodies as functional internal images of enzymes active sites: production of a catalytic antibody with a cholinesterase activity. Proc. Natl. Acad. Sci. USA 90, 8876–8880.PubMedCrossRefGoogle Scholar
  25. 25.
    Paul, S., Voile, D. J., Beach, C. M., Johnson, D. R., Powell, M. J., and Massey, R. J. (1989) Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody. Science 244, 1158–1162.PubMedCrossRefGoogle Scholar
  26. 26.
    Li, L., Kaveri, S., Tyutyulkova, S., Kazatchkine, M., and Paul, S. (1994) Catalytic activity of anti-thyroglobulin antibodies. J. Immunol. 154, 3328–3332.Google Scholar
  27. 27.
    Paul, S. (1994) Catalytic activity of anti-ground state antibodies, antibody subunits and human autoantibodies. Appl Biochem. Biotechnol. 47, 241–255.PubMedCrossRefGoogle Scholar
  28. 28.
    Shuster, A. M., Gololobov, G. V., Kvashuk, O. A., Bogomolova, A. E., Smirnov, I. V., and Gabibov, A. G. (1992) DNA hydrolyzing autoantibodies. Science. 256, 665–669.PubMedCrossRefGoogle Scholar
  29. 29.
    Shuster, A. M., Gololobov, G. V., Kvashuk, D. A., and Gabibov, A. G. (1991) Antiidiotypic and natural catalytically active antibodies. Mol. Biol. (Mosk) 25, 593–602.Google Scholar

Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Howard Amital
    • 1
  • Ilan Tur-Kaspa
    • 2
  • Zeev Tashma
    • 2
  • Israel Hendler
    • 2
  • Yehuda Shoenfeld
    • 1
  1. 1.Department of Medicine ‘B’ and Research Unit of Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael
  2. 2.Israeli Defense Forces-Medical CorpsTel-AvivIsrael

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