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Techniques for Conjugation of Synthetic Peptides to Carrier Molecules

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Peptide Analysis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 36))

Abstract

There are two common purposes for conjugation of peptides. The most common is induction of humoral immunity. This is the production of antibodies capable of binding to the peptide immunogen. The antibodies are elaborated by plasma cells, which are terminally differentiated B-lymphocytes. However, in order for immunity to be successfully induced in a secondary anamnestic response, the immunogen must also react with T-lymphocytes. Many peptides contain B-cell epitopes, but not T-cell epitopes. In immunological terms, these peptides and other such molecules are called haptens. Coupling these molecules to a large carrier protein containing T-cell epitopes allows the induction of a B-cell response to the entire immunogen, including the peptide (1). New synthetic peptides offer promise as vaccines.

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Authors and Affiliations

  1. Cytogen, Princeton, NJ

    J. Mark Carter

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  1. J. Mark Carter
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Editor information

Editors and Affiliations

  1. University of Florida, Gainesville, FL

    Ben M. Dunn

  2. Bachem Bioscience, King of Prussia, PA

    Michael W. Pennington

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© 1994 Humana Press Inc., Totowa, NJ

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Carter, J.M. (1994). Techniques for Conjugation of Synthetic Peptides to Carrier Molecules. In: Dunn, B.M., Pennington, M.W. (eds) Peptide Analysis Protocols. Methods in Molecular Biology, vol 36. Humana Press. https://doi.org/10.1385/0-89603-274-4:155

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  • DOI: https://doi.org/10.1385/0-89603-274-4:155

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-274-3

  • Online ISBN: 978-1-59259-523-5

  • eBook Packages: Springer Protocols

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