Techniques for Conjugation of Synthetic Peptides to Carrier Molecules

  • J. Mark Carter
Part of the Methods in Molecular Biology book series (MIMB, volume 36)

Abstract

There are two common purposes for conjugation of peptides. The most common is induction of humoral immunity. This is the production of antibodies capable of binding to the peptide immunogen. The antibodies are elaborated by plasma cells, which are terminally differentiated B-lymphocytes. However, in order for immunity to be successfully induced in a secondary anamnestic response, the immunogen must also react with T-lymphocytes. Many peptides contain B-cell epitopes, but not T-cell epitopes. In immunological terms, these peptides and other such molecules are called haptens. Coupling these molecules to a large carrier protein containing T-cell epitopes allows the induction of a B-cell response to the entire immunogen, including the peptide (1). New synthetic peptides offer promise as vaccines.

Keywords

Vortex Foam DMSO Microbial Degradation Disulfide 

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Copyright information

© Humana Press Inc., Totowa, NJ 1994

Authors and Affiliations

  • J. Mark Carter
    • 1
  1. 1.CytogenPrinceton

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