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Acid Cleavage/Deprotection in Fmoc/tBiu Solid-Phase Peptide Synthesis

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Peptide Synthesis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 35))

Abstract

In general, a solid-phase peptide synthesis (SPPS) consists of the assembly of a protected peptide chain on a polymeric support (=synthetic step) and the subsequent cleavage/deprotection to release the crude, depro-tected peptide from the solid support (=cleavage step). Usually, these two steps are followed by chromatographic purification of the crude peptide (see Chapters 1, Chapter 2, Chapter 4, and Chapter 5, PAP). The techniques to synthesize protected peptides or peptide fragments are discussed in Chapter 14. This chapter describes exclusively the cleavage step in the Fmoc/tBu-SPPS with TFA as cleavage reagent leading to fully deprotected peptides (see Note 1) (1). All of the cleavage procedures that are exemplified in Section 3. of this chapter hold for peptides synthesized under the following conditions

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References

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Author information

Authors and Affiliations

  1. Bachem Feinchemikalien AG, Bubendorf, Switzerland

    Fritz Dick

Authors
  1. Fritz Dick
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Editor information

Editors and Affiliations

  1. Bachem Bioscience, King of Prussia, PA, USA

    Michael W. Pennington

  2. University of Florida, Gainesville, FL, USA

    Ben M. Dunn

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© 1994 Humana Press Inc.

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Dick, F. (1994). Acid Cleavage/Deprotection in Fmoc/tBiu Solid-Phase Peptide Synthesis. In: Pennington, M.W., Dunn, B.M. (eds) Peptide Synthesis Protocols. Methods in Molecular Biology, vol 35. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-273-6:63

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  • DOI: https://doi.org/10.1385/0-89603-273-6:63

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-273-6

  • Online ISBN: 978-1-59259-522-8

  • eBook Packages: Springer Protocols

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